Multi-index disease combined detection microfluidic device

A microfluidic device and combined detection technology, applied in the field of life medical detection and diagnosis, can solve the problems of complex detection process, high detection cost, low detection efficiency, etc., and achieve the effect of high capture efficiency and simple operation.

Active Publication Date: 2019-04-19
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In traditional ELISA detection, it is often necessary to rely on large-scale biomedical diagnostic instruments, which are expensive, complex detection process, high detection cost, long detection time and low efficiency, which is not conducive to the clinical promotion and pop

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  • Multi-index disease combined detection microfluidic device
  • Multi-index disease combined detection microfluidic device
  • Multi-index disease combined detection microfluidic device

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Embodiment Construction

[0066] Below will combine in the present invention Figure 1 to Figure 5 , clearly and completely describe the technical solutions in the embodiments of the present invention, obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. The following description of an exemplary embodiment is merely illustrative in nature and is by no means intended to limit the invention and its application or uses. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0067] The relative arrangements of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise.

[0068] At the same time, it should be understood that, for the convenience of description, the sizes...

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Abstract

The invention discloses a multi-index disease combined detection microfluidic device, which comprises a microfluidic reaction chip, a microfluidic storage chip, a direct current motor driving module,a reaction chip fluid control module, a storage release fluid control module, a temperature control module, and a CCD detection module. Through the cooperation between the reaction chip fluid controlmodule, the storage release fluid control module, the temperature control module, and the microfluidic reaction chip, a sample and a sample diluting solution are mixed; microsphere surface coated antibodies capture target cell specific antigens, non-specific adsorbed cell antigens on the microsphere surface are washed off, target cell antigens specifically absorbed on the microsphere surface capture enzyme labeled antibodies, exciting liquids (A and B) are mixed, enzymes specifically adsorbed on the microsphere surface catalyze the chemiluminescence of the exciting liquids; the CCD detection module detects and collects the chemiluminescence signals of the microsphere surface, and multiple index antigens in a serum sample are captured and detected automatically.

Description

technical field [0001] The invention relates to the field of life medical detection and diagnosis, and is a multi-index disease joint detection microfluidic chip and detection device based on the enzyme-linked immunosorbent assay method and the principle of chemiluminescence. The microfluidic detection device can be used for the simultaneous detection of multiple disease markers, for example, for the detection of five indicators of prenatal and postnatal care: toxoplasma gondii (TOX), rubella (RUB), giant cell (CMV), herpes ( HSV-1), herpes (HSV-2) and other five IgG (immunoglobulin) antibody levels. technical background [0002] Enzyme-linked immunosorbent assay (ELISA) has been widely used in various disease diagnosis based on protein detection. The principle of ELISA is through the specificity of the bond between the antigen (ie protein) and the antibody, through the color change with the enzyme Or a luminescent reaction that proves the presence of a particular protein. ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/543
CPCB01L3/5027G01N33/543
Inventor 邱宪波余盛达李逸琛
Owner BEIJING UNIV OF CHEM TECH
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