Three-dimensional chromatographic separation system on basis of two-position ten-way valve

Abstract

The invention provides an all-online three-dimensional high-performance liquid chromatographic separation system. Procedures from sample pretreatment to sample separation until sample analysis can beautomated by the aid of the all-online three-dimensional high-performance liquid chromatographic separation system. The all-online three-dimensional high-performance liquid chromatographic separationsystem comprises a high-performance liquid chromatographic gradient pump A, a high-performance liquid chromatographic gradient pump B, a high-performance liquid diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a distillate collector, a liquid chromatographic separation column array, a detector, a two-position ten-way valve and connecting pipelines. The all-online three-dimensional high-performance liquid chromatographic separation system has the advantages that separation states of a previous dimension can be converted into separation states of a next dimension under switching effects of the two-position ten-way valve, so that three-dimensional chromatographic separation can be implemented; chromatographic separation columns of the various dimensions can be selected by the liquid chromatographic separation column array, three-dimensional chromatographic separation can be monitored and controlledin an all-online manner on the basis of the same gradient elution system and the same detector, and accordingly the cleanliness of enrichment columns and separation columns can be controlled; separation of the various dimensions is connected by the enrichment columns, and the diluent pump can assist in enriching or trapping compounds; different types of chromatographic stationary phase and mobilephase are selected to be combined with one another, and accordingly monomeric compounds in samples of complicated systems with high separation difficulty can be efficiently separated from one another.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation and relates to a three-dimensional liquid chromatography separation system. Background technique [0002] With the development of life science, material science and environmental science, the separation and analysis of complex sample systems has become an important research direction of analytical chemistry. Since the 1970s, both the basic theory of chromatography and the performance of instruments have been greatly improved and improved. Although the particle size reduction of chromatographic fillers and the improvement of the physical and chemical properties of the stationary phase surface have greatly improved the resolving power of liquid chromatography columns, the real separation ability of liquid chromatography has not been significantly improved accordingly. Due to the complexity of biological products, their analysis often requires a combination ...

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Problems solved by technology

However, the inlet and outlet of the enrichment column array in this system are fixed. When the enrichment column is used as a sample loading column, the flow direction of the eluent is consistent with the flow direction of the mobile phase when the sample was originally enric

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