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A kind of fungal laccase expressing strain and its construction method and application

A technology of fungal laccase and strain, applied in the field of fungal laccase expression strain and its construction, can solve the problems of difficulty in meeting industrialization requirements, high cost, low yield and the like

Active Publication Date: 2022-04-26
ANHUI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although some fungal laccase genes have been successfully recombinantly expressed, the heterologous expression of fungal laccase has the disadvantages of low yield, long fermentation cycle and high cost, and it is difficult to meet the requirements of industrialization.

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  • A kind of fungal laccase expressing strain and its construction method and application
  • A kind of fungal laccase expressing strain and its construction method and application
  • A kind of fungal laccase expressing strain and its construction method and application

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Embodiment Construction

[0075] The present invention transforms Trameteshirsuta AH28-2, a laccase high-yielding bacterial strain screened and isolated by our laboratory by genetic means, that is, the heat shock protein ThHspA1 is overexpressed in the bacterial strain, and bacterial strain Trameteshirsuta AH28-2 (ThHspA1 -5) The production of laccase can be increased by 2 times under the condition of adding 1 / 10 of the initial dose of inducer, and the production of laccase with this strain has the advantages of high efficiency and environmental protection.

[0076] The classification of Trametes sp.AH28-2 is named: Trametes sp.AH28-2, deposit unit: China Collection Center for Typical Cultures, address: China. Wuhan. Wuhan University, deposit date: November 29, 2005, deposit number : CCTCC NO: M205134.

[0077] Trametes hirsuta AH28-2 (ThHspA1-5), a fungal laccase expression strain of the present invention, depository unit: China Typical Culture Collection Center, address: China. Wuhan. Wuhan Universit...

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Abstract

The invention discloses a fungal laccase expression strain and its construction method and application. The laccase high-yield strain Trametes AH28-2 is transformed by genetic means, that is, the heat shock protein ThHspA1 is overexpressed in the strain, and after transformation The strain Trametes hirsuta AH28‑2 (ThHspA1‑5) can increase the production of laccase by 2 times under the condition of adding 1 / 10 of the initial dose of inducer, and the production of laccase by this strain has the advantages of high efficiency and environmental protection.

Description

technical field [0001] The invention relates to a fungal laccase expression strain, a construction method and application thereof. Background technique [0002] Fungal laccase (laccase, EC1.10.3.2) is a class of copper-containing polyphenol oxidase, which is widely distributed in higher filamentous fungi, especially basidiomycetes, and can catalyze the oxidation of hundreds of phenolic and nonphenolic compounds. , while reducing oxygen molecules to water; in the presence of small molecule mediator substances such as methyl syringate, the substrates of fungal laccase can be further broadened. In recent years, with the society's appeal and pursuit of sustainable and environmentally friendly catalysis, fungal laccases have a wide substrate spectrum, and O 2 as the electron acceptor by-product is H 2 The catalytic advantages of O and other aspects are gradually reflected, and the research on fungal laccases has therefore attracted extensive attention from scientific researcher...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/80C12N9/02C12R1/645
CPCC12N9/0061C12N15/80C12Y110/03002C12R2001/645C12N1/145
Inventor 方泽民赵文梦张艳凤王晶晶肖亚中
Owner ANHUI UNIVERSITY