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Extracting method for soluble gadus protein

A technology of cod protein and extraction method, applied in the field of extraction of soluble cod protein, can solve problems such as poor solubility of cod protein, application limitation and the like

Inactive Publication Date: 2019-04-26
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the isoelectric point precipitation method is usually used to recover the protein in the by-products of aquatic products processing, but the cod protein extracted by "alkali-soluble acid precipitation" has poor solubility, which greatly limits its application in the food industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1. Cut the cod meat into pieces, add water according to the ratio of material to liquid 1:10 (w / v, g / ml), beat at 200rpm for 20 minutes, and obtain the fish suspension; among them, whether the cod meat is cut into pieces does not affect the quality of the obtained cod protein , the purpose of cutting into pieces is to be able to place the cod meat in a beater for beating;

[0026] S2. Use NaOH to adjust the pH value of the fish suspension obtained in step S1 to 9.0, and stir at 40° C. and 50 rpm for 3 hours to obtain suspension 1;

[0027] S3, centrifuge the suspension 1 obtained in step S2 at 10000r / min for 10min, and take the supernatant;

[0028] S4. Use HCl to adjust the pH of the supernatant obtained in step S3 to 4.5, and let it stand for 0.5 h to obtain a protein dispersion;

[0029] S5. Centrifuge the protein dispersion obtained in step S4 at 5000r / min for 30min to collect the precipitate;

[0030] S6, add the precipitate obtained in step S5 to water (accordi...

Embodiment 2

[0035] S1, take the cod steak and cut into pieces, add water according to the ratio of material to liquid 1:10 (w / v, g / ml), beat at 10000rpm for 2min, and obtain the fish suspension;

[0036] S2. Use NaOH to adjust the pH value of the fish suspension obtained in step S1 to 10.0, and stir at 60° C. and 500 rpm for 1 hour to obtain suspension 1;

[0037] S3, centrifuge the suspension 1 obtained in step S2, centrifuge at 9000r / min for 20min, and take the supernatant;

[0038] S4. Use HCl to adjust the pH of the supernatant obtained in step S3 to 4.0, and let it stand for 1.0 h to obtain a protein dispersion;

[0039] S5, centrifuge the protein dispersion obtained in step S4 at 7000r / min for 20min to collect the precipitate;

[0040] S6. Add 10 times the weight of water to the precipitate obtained in step S5, adjust the pH to 7.5, disperse and redissolve at 600 rpm, and obtain a protein suspension;

[0041] S7, centrifuge the protein suspension obtained in step S6 at 9000r / min f...

Embodiment 3

[0045] S1, take the cod steak and cut into pieces, add water according to the ratio of material to liquid 1:15 (w / v, g / ml), beat at 600rpm for 10min, and obtain the fish suspension;

[0046] S2. Use NaOH to adjust the pH value of the fish suspension obtained in step S1 to 11.0, and stir at 50° C. and 300 rpm for 2 hours to obtain suspension 1;

[0047] S3, centrifuge the suspension 1 obtained in step S2 at 8000r / min for 40min, and take the supernatant;

[0048] S4. Use HCl to adjust the pH of the supernatant obtained in step S3 to 4.5, and let it stand for 0.2 hours to obtain a protein dispersion;

[0049] S5. Centrifuge the protein dispersion obtained in step S4 at 10,000 r / min for 5 minutes to collect the precipitate;

[0050] S6, add the precipitate obtained in step S5 to water (according to 1:7 weight ratio), adjust the pH to 7.5, disperse and redissolve at 2000rpm, and obtain a protein suspension;

[0051] S7, centrifuge the protein suspension obtained in step S6 at 100...

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Abstract

The invention discloses an extracting method for soluble gadus protein. The method comprises the following steps: S1, taking codfish, adding water and pulping, wherein a solid-liquid ratio is 1:5-1:15, to obtain fish suspension liquid; S2, adjusting a pH (Potential Hydrogen) value of the fish suspension liquid to be 9.0-11.0, and stirring for 1-3 hours in 40-60 DEG C at a speed of 50-500 rpm to obtain suspension liquid 1; S3, centrifuging the suspension liquid 1, and taking supernatant; S4, adjusting the pH of the supernatant to be 4.0-5.0, and still standing for 0.1-1 h to obtain protein dispersion liquid; S5, centrifuging the protein dispersion liquid and collecting a precipitate; S6, adding water to the precipitate, adjusting a pH to be 7.0-7.5, stirring at a speed of 300-2000 rpm, dispersing and redissolving to obtain protein suspension liquid; S7, centrifuging the protein suspension liquid, and taking supernatant; and S8, drying the supernatant to obtain the soluble gadus protein.The obtained soluble gadus protein is high in extraction ratio, and good in solubleness.

Description

technical field [0001] The invention relates to the field of aquatic protein processing, in particular to a method for extracting soluble cod protein. Background technique [0002] Cod is an ocean deep-water fish widely distributed in the North Pacific Ocean, and is one of the fishes with the largest annual catch in the world. my country is mainly distributed in the Bohai Sea, the Yellow Sea and the northern part of the East China Sea. The protein content of cod meat is as high as 20%, and the contained amino acids are complete, so it has high use and economic value. At present, the isoelectric point precipitation method is usually used to recover the protein in the by-products of aquatic products processing, but the cod protein extracted by "alkali-soluble acid precipitation" has poor solubility, which greatly limits its application in the food industry. Therefore, how to prepare soluble cod protein is an urgent problem to be solved in expanding its application in the foo...

Claims

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Application Information

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IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 吴超马武超杜明延新宇徐献兵秦磊于翠平王震宇
Owner DALIAN POLYTECHNIC UNIVERSITY
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