Lactobacillus plantarum and its use in lowering blood lipid, liver function index, uric acid and anti-inflammation

A technology for the use of Lactobacillus plantarum, which is applied in the field of Lactobacillus plantarum strains to prevent and/or treat obesity-related diseases, and can solve problems such as inability to meet the needs of relief

Active Publication Date: 2022-04-29
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, strains with a single effect cannot meet the relief needs of various symptoms caused by obesity, and there is still a need to develop strains with compound effects in the field of medicine for prevention and / or treatment, so as to more effectively target obesity-related symptoms at the same time. The disease achieved the effect of improvement

Method used

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  • Lactobacillus plantarum and its use in lowering blood lipid, liver function index, uric acid and anti-inflammation
  • Lactobacillus plantarum and its use in lowering blood lipid, liver function index, uric acid and anti-inflammation
  • Lactobacillus plantarum and its use in lowering blood lipid, liver function index, uric acid and anti-inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1: collection, separation and purification of strains

[0089] Visit farmers and families engaged in farming in Yilan, Hualien, Taitung and other places, and observe the elderly farmers who are over 75 years old and can still be self-sufficient and cultivate flexibly. It is inferred that the long-term intake of food containing lactic acid bacteria means that long-term exposure to strains with unique prebiotic effects will help elderly farmers maintain good physiological function. Therefore, collect samples that may contain lactic acid bacteria everywhere, store the samples in sealed sterile bags, refrigerate them in the mobile refrigerator and take them back to the laboratory to isolate and purify the probiotic strains contained in the samples. Firstly, prepare the culture medium containing different nutrient sources to screen different kinds of lactic acid strains in the sample. For example, Mrs agar, PCA agar, M17 agar, Rogosa agar, TOS MUP agar, Mrs agar with 3% ...

Embodiment 2

[0091] Example 2: gene analysis and identification of strains

[0092] Firstly, the DNA of gkm3 was collected for sequencing, and the strains were identified by gene comparison. Gkm3 was activated by scribing, and a single colony was inoculated into the culture medium with an inoculation ring. Fresh bacteria were collected the next day, and the strain gDNA was extracted by CTAB method. The gDNA quality was confirmed by 0.6% agar gel electrophoresis. After concentration, the whole genome of the strain was analyzed by the third generation sequencing technology platform pacbio rsii. According to the analysis results of the whole genome, the length of gkm3 whole genome is 3076381bp and contains a 49415bp plasmid (SEQ ID No: 1). The whole genome sequences of gkm3 were compared in the whole genome database, and the sequences of PHEs (SEQ ID No: 2) and recn (SEQ ID No: 3) genes of gkm3 were obtained. Then, the sequences of the PHEs gene (SEQ ID No: 2) of gkm3 were compared in the NCBI da...

Embodiment 3

[0099] Example 3: strain culture

[0100] The effects of different culture conditions on the yield of gkm3 were studied. The carbon source and nitrogen source in the fermentation medium of gkm3 were tested by one factor test.

[0101] Firstly, starting from the demand of carbon source, compare the number of gkm3 colonies and IFN to peripheral leukocyte lymphocytes under the conditions of eight carbon sources: glucose, sucrose, lactose, fructose, mannose, sorbitol, glycerol and molasses- γ Induction ability to select carbon sources with high yield and high physiological activity of gkm3. Add 3wt% of the above eight carbon sources relative to the total weight of the MRS medium, and set the culture temperature at about 37 ℃ and the rotating speed at about 20rpm. The better four carbon sources and the number of viable colonies in the experimental results are shown in Table 2 below. The results show that the highest number of colonies cultured with sucrose is 4.2x10 9 CFU / ml, compare...

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Abstract

The present invention provides a Lactobacillus plantarum and its use for lowering blood fat, liver function index, uric acid and anti-inflammation, specifically a Lactobacillus plantarum GKM3 with a specific gene sequence, which has acid resistance, alkali resistance and / or cell adsorption ability, And it can achieve the effect of eliminating body fat, lowering blood fat, reducing hepatomegaly, lowering liver function index, anti-inflammation, lowering uric acid, improving allergies and / or lowering blood sugar. Accordingly, the present invention provides a new type of Lactobacillus plantarum, and further develops the medical application of the Lactobacillus plantarum as an alternative choice for preventing and / or treating obesity-related diseases in the field.

Description

technical field [0001] The invention relates to a Lactobacillus germ, a composition containing it, a culture method and its application; More specifically, it relates to a Lactobacillus germ strain containing PHEs gene, recn gene and plasmid, which can be used to prevent and / or treat obesity related diseases by eliminating body fat, reducing blood lipid, reducing hepatomegaly, reducing liver function index, anti-inflammatory, reducing uric acid, improving allergy and / or reducing blood sugar. Background technology [0002] Obesity [0003] With the change of life style, especially the change of eating habits, the obesity rate increases, which makes obesity a serious health problem in modern countries. The World Health Organization (who) has listed obesity as the most important subject of public health and preventive medicine in the 21st century. According to the definition of obesity by who, BMI (body mass index) is taken as the standard, and BMI is greater than 30kg / m 2 It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61P1/16A61P3/06A61P19/06A61P29/00A23L2/00A23C9/12A23C9/152C12R1/25
CPCA23C9/12A23C9/152A23K10/18A23L2/00A23L33/135C12N1/20A61K2035/115C12R2001/25C12N1/205
Inventor 陈劲初陈炎炼许胜杰林珊林诗伟李丽雅吴思颖陈彦博王启宪侯毓欣石仰慈林静雯陈雅君江佳琳
Owner GRAPE KING
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