Method for effectively anchoring candidate gene region of peanut quantitative traits

Abstract

The invention relates to the field of biotechnology, and in particular to a method for effectively anchoring a candidate gene region of a quantitative trait. The method is co-localized with a genome-wide association study and a quantitative trait localization method to achieve efficient anchoring of the quantitative trait candidate gene regions. The method achieves effective anchoring of the candidate gene region of the peanut quantitative trait by co-localization with the genome-wide association study and the quantitative trait localization. The method is exemplified by quantitative granule weight, firstly, through a simplified genome sequencing technology, the genome-wide SNP marker development of 165 cultivar peanut core collections in China is carried out; based on a principle of linkage disequilibrium, combined with peanut 100-fruit weight and 100-kernel weight breeding value, the related gene regions related to peanut weight traits are associated; in the previous reports, the QTLmapping results of the grain weight traits are converted to the corresponding genomic physical locations, the co-localization region of the two methods can be obtained, and the method can effectivelyand accurately realize the localization of the genome-wide particle weight-related trait genes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for effectively anchoring candidate gene regions of peanut quantitative traits. Background technique [0002] The peanut cultivar (Arachis hypogaea L.) is an important oil crop worldwide. China is the largest producer of cultivated peanuts, accounting for more than 40% of total global production. However, it still cannot satisfy the national demand for peanuts. Therefore, cultivating high-quality and high-yielding peanut varieties is still a long-term and arduous task. With the development of biotechnology, genetic breeding has avoided the time-consuming and labor-intensive shortcomings of traditional breeding. The premise of genetic breeding is to accurately anchor the relevant candidate gene regions of important quantitative traits such as yield and quality. The aggregation breeding based on favorable alleles related to each trait has become the most concerned hotspot ...

AI Technical Summary

Problems solved by technology

To sum up, there is still a lack of in-depth analysis of the characteristics and interrelationships of phenotype and genotype variation in peanuts.

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