A retinitis pigmentosa screening kit
A retinal pigment and screening technology, which is applied in the field of gene point mutations and point mutations, can solve the problems of incomplete mutation sites and missed diagnosis of RP, and achieve reliable and specific effects
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[0025] Example Kit of the present invention and method of use
[0026] All components, contents and use methods in the kit of the present invention are as follows:
[0027] 1. PCR amplification reagents (50 copies):
[0028] The PCR amplification reagent is used to amplify a DNA sequence where the mutation site is located, and its composition is shown in Table 1.
[0029] Table 1 PCR amplification reagents
[0030] component concentration volume PCR mix 2× 600μl primer pair 10μM 100μl pure water 2ml
[0031] The PCR mixture in Table 1 includes Taq enzyme, dNTP, magnesium ions and other components required for conventional PCR; the primer pair information is shown in Table 2.
[0032] Table 2 Primers used for CRB1 gene amplification
[0033]
[0034] 2. CRB1 gene variant typing detection reagent (50 copies):
[0035] The reagent included the components shown in Table 3.
[0036] Table 3 Detection reagents for CRB1 gene variant...
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