Method and system for identifying Chinese population epithelial cell body fluid spot tissue source from gene level

An epithelial cell and blotting technology, which is applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as false positives and affect the interpretation of identification results.

Inactive Publication Date: 2019-05-10
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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Problems solved by technology

[0005] The above-mentioned saliva, vaginal secretions, and skin contact spots are the focus of this research. These three body fluid spots have certain commonalities: they all contain cells of epithelial origin, and the cell functions are similar, and the expressed proteins also hav

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  • Method and system for identifying Chinese population epithelial cell body fluid spot tissue source from gene level
  • Method and system for identifying Chinese population epithelial cell body fluid spot tissue source from gene level
  • Method and system for identifying Chinese population epithelial cell body fluid spot tissue source from gene level

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Embodiment Construction

[0070] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, and Not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0071] The samples used in the examples of the present invention are all from unrelated individuals, among which the skin wipes and saliva samples were collected from a university in Beijing, covering students and teachers, the ratio of male to female volunteers was 1:1, and the vaginal secretion samples were collected from women in a hospital. obstetrics. The collected above-mentioned body fluid sample...

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Abstract

The invention provides a method and system for identifying a Chinese population epithelial cell body fluid spot tissue source from the gene level. The method comprises the steps of utilizing a first PCR amplification primer set for performing first amplification on body fluid spot DNA, and obtaining a first amplification PCR product, wherein the first PCR amplification primer set is composed of nine pairs of amplification primers corresponding to nine genes of CYP2B7P1, MUC4, L.cri, HTN3, STATH, MUC7, LCE2B, DCD and CCL27 one to one; according to the gene expression condition in the first amplification PCR product, whether or not at least one of vaginal secretion, saliva and skin is contained in body fluid spots is judged. According to the scheme, the Chinese population body fluid tissue source can be effectively determined from the gene level, the step for identifying the body fluid or spot tissue source thereof at a criminal investigation site is greatly simplified, and the accuratescientific basis can be supplied to case character clearing, conviction and sentencing.

Description

technical field [0001] The present invention relates to a method and a system for the source of human body fluid tissue in a population, in particular to a method and a system for identifying the source of epithelial cell body fluid spot tissue in a Chinese population at the genetic level. Background technique [0002] Body fluid stains are common biological evidence at crime scenes, which can be analyzed using deoxyribonucleic acid (DNA) identification technology to identify or exclude suspects. In recent years, with the deepening of the reform of the litigation system, only obtaining DNA typing from biological evidence to determine the individual source is increasingly unable to meet the integrity requirements of the evidence, and this single result has also caused some controversy. The identification of traces is precisely an important part of the controversy. Traditional body fluid spot detection methods, such as salivary amylase test, mainly judge the existence of the ...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
Inventor 王冲赵禾苗孙辉李万水孟庆振陈静徐秀兰徐珍凃政
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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