Identification method of Minqing Maojiao Chicken breed

A kind of chicken and genotyping technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., which can solve the problems of different degrees of difficulty, different distribution frequencies of specific markers, and different primer specificities And other issues

Pending Publication Date: 2021-04-23
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason may be that the distribution frequency of specific markers in different populations is different, the difficulty of accurate replication of gene segments at different sites is different, or the specificity of primers used for amplification is different.

Method used

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  • Identification method of Minqing Maojiao Chicken breed
  • Identification method of Minqing Maojiao Chicken breed
  • Identification method of Minqing Maojiao Chicken breed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0156] Example 1 Simplified Genome Sequencing Screening for Species-Specific SNPs / INDELs Markers in Minqing Maojiao Chicken

[0157] (one)

[0158] Samples for sample: including Minqing Maojiao Chicken (20), Jining Hundred Day Chicken (20), Langya Chicken (20), Shouguang Chicken (20), Wenshang Reed Chicken (20), Rugao Yellow Chicken (20), Minqing Maojiao Chicken (20), Taihu Chicken (20), Liyang Chicken (20), Xuhai Chicken (20), Longsheng Phoenix Chicken (20), Langshan Chicken ( 20), Huaibei Maji (20), half male and half female for each breed. 0.5-1.0ml of blood was collected from the wing vein, placed in an anticoagulant tube, and stored at -70°C.

[0159] Genomic DNA was extracted by the CTAB method, and the genomic DNA was digested with restriction enzymes EcoRI and NIaIII, and then sequence adapters were added to construct a small fragment library for GBS simplified genome sequencing.

[0160] In order to check the efficiency of enzyme digestion and facilitate subsequent...

Embodiment 2

[0219] Example 2 A method for effectively identifying Minqing Maojiao chicken

[0220] The method includes, using the following specific genetic loci for identification:

[0221] MQ_tag1 is located at 172713037 of chicken chromosome 1, the second intron of gene FREM2, and the genotype of Minqing Maojiao chicken is -- (the mutant genotype is the deletion fragment TTTTG);

[0222] MQ_tag2 is located at chicken No. 2 chromosome 36785616, between the gene ZNF385D and UBE2E2, Minqing Maojiao chicken is the insertion genotype, and the genotype is TT;

[0223] MQ_tag3 is located at 90080648 on chicken chromosome 2, the second intron of gene CARMIL1, and the genotype of Minqing Maojiao chicken is TT;

[0224] MQ_tag4 is located at chicken chromosome 3 71462156, the 8th intron of USP45 gene, and the genotype of Minqing Maojiao chicken is TT;

[0225] MQ_tag5 is located at chicken No. 4 chromosome 33359244, between the SH3D19 gene and the GATB gene, and the genotype of Minqing Maojiao...

Embodiment 3

[0236] Example 3 A method for effectively identifying Minqing Maojiao chicken

[0237] The method includes using the following specific genetic loci for identification: MQ_tag2 is located at chicken No. 2 chromosome 36785616, between the gene ZNF385D and UBE2E2, and the Minqing Maojiao chicken is the insertion genotype, and the genotype is TT;

[0238]MQ_tag3 is located at 90080648 on chicken chromosome 2, the second intron of gene CARMIL1, and the genotype of Minqing Maojiao chicken is TT;

[0239] MQ_tag5 is located at chicken No. 4 chromosome 33359244, between the SH3D19 gene and the GATB gene, and the genotype of Minqing Maojiao chicken is AA;

[0240] MQ_tag7 is located at 15730043 on chicken chromosome 6, the first intron of SAMD8 gene, and the genotype of Minqing Maojiao chicken is GG;

[0241] MQ_tag8 is located at 23125070 on chicken chromosome 7, between the TNS1 gene and the IGFBP5 gene, and the genotype of Minqing Maojiao chicken is GG;

[0242] MQ_tag9 is locate...

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Abstract

The invention relates to an identification method of poultry breeds, and particularly relates to an identification method of the Minqing Maojiao Chicken breed. On the basis of obtaining a group of specific genetic loci of the Minqing Maojiao Chicken through screening, the obtained genetic loci are further subjected to optimizing and screening, the specific loci with high identification rate of Minqing Maojiao Chicken are obtained through screening, and the specific loci obtained through screening can be used for combined identification, so that the accuracy is higher, and the obtained result is more reliable.

Description

technical field [0001] The invention relates to an identification method of poultry breeds, in particular to an identification method of Minqing Maojiao chicken breeds. Background technique [0002] Minqing Maojiao Chicken, commonly known as Minqing Local Chicken, Minqing Local Chicken, Maojiao Chicken, and Phoenix-tailed Chicken, is a dual-purpose local breed. The central production areas of Minqing Maojiao Chicken are Baizhong Town and Bandong Town in Minqing County, Fujian Province. In 2009, "Minqing Maojiao Chicken" was approved by the Poultry Professional Committee of the Ministry of Agriculture and recognized as a breed of livestock and poultry genetic resources; in 2014, "Minqing Maojiao Chicken" was included in the "National List of Animal Genetic Resources Protection". In recent years, with the efforts of all walks of life, the brand of Minqing Maojiao chicken has had a small influence. However, due to the low threshold of breeding technology, other local chicken ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 马猛曲亮李尚民胡玉萍卢建窦套存
Owner JIANGSU INST OF POULTRY SCI
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