Identification method of Minqing Maojiao Chicken breed
A kind of chicken and genotyping technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., which can solve the problems of different degrees of difficulty, different distribution frequencies of specific markers, and different primer specificities And other issues
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Embodiment 1
[0156] Example 1 Simplified Genome Sequencing Screening for Species-Specific SNPs / INDELs Markers in Minqing Maojiao Chicken
[0157] (one)
[0158] Samples for sample: including Minqing Maojiao Chicken (20), Jining Hundred Day Chicken (20), Langya Chicken (20), Shouguang Chicken (20), Wenshang Reed Chicken (20), Rugao Yellow Chicken (20), Minqing Maojiao Chicken (20), Taihu Chicken (20), Liyang Chicken (20), Xuhai Chicken (20), Longsheng Phoenix Chicken (20), Langshan Chicken ( 20), Huaibei Maji (20), half male and half female for each breed. 0.5-1.0ml of blood was collected from the wing vein, placed in an anticoagulant tube, and stored at -70°C.
[0159] Genomic DNA was extracted by the CTAB method, and the genomic DNA was digested with restriction enzymes EcoRI and NIaIII, and then sequence adapters were added to construct a small fragment library for GBS simplified genome sequencing.
[0160] In order to check the efficiency of enzyme digestion and facilitate subsequent...
Embodiment 2
[0219] Example 2 A method for effectively identifying Minqing Maojiao chicken
[0220] The method includes, using the following specific genetic loci for identification:
[0221] MQ_tag1 is located at 172713037 of chicken chromosome 1, the second intron of gene FREM2, and the genotype of Minqing Maojiao chicken is -- (the mutant genotype is the deletion fragment TTTTG);
[0222] MQ_tag2 is located at chicken No. 2 chromosome 36785616, between the gene ZNF385D and UBE2E2, Minqing Maojiao chicken is the insertion genotype, and the genotype is TT;
[0223] MQ_tag3 is located at 90080648 on chicken chromosome 2, the second intron of gene CARMIL1, and the genotype of Minqing Maojiao chicken is TT;
[0224] MQ_tag4 is located at chicken chromosome 3 71462156, the 8th intron of USP45 gene, and the genotype of Minqing Maojiao chicken is TT;
[0225] MQ_tag5 is located at chicken No. 4 chromosome 33359244, between the SH3D19 gene and the GATB gene, and the genotype of Minqing Maojiao...
Embodiment 3
[0236] Example 3 A method for effectively identifying Minqing Maojiao chicken
[0237] The method includes using the following specific genetic loci for identification: MQ_tag2 is located at chicken No. 2 chromosome 36785616, between the gene ZNF385D and UBE2E2, and the Minqing Maojiao chicken is the insertion genotype, and the genotype is TT;
[0238]MQ_tag3 is located at 90080648 on chicken chromosome 2, the second intron of gene CARMIL1, and the genotype of Minqing Maojiao chicken is TT;
[0239] MQ_tag5 is located at chicken No. 4 chromosome 33359244, between the SH3D19 gene and the GATB gene, and the genotype of Minqing Maojiao chicken is AA;
[0240] MQ_tag7 is located at 15730043 on chicken chromosome 6, the first intron of SAMD8 gene, and the genotype of Minqing Maojiao chicken is GG;
[0241] MQ_tag8 is located at 23125070 on chicken chromosome 7, between the TNS1 gene and the IGFBP5 gene, and the genotype of Minqing Maojiao chicken is GG;
[0242] MQ_tag9 is locate...
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