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Integrated centrifuge

A centrifuge and chemical reaction technology, applied in the field of biomedicine, can solve the problems of decreased detection throughput and contamination of samples to be tested, and achieve the effect of broad market prospects.

Active Publication Date: 2019-05-14
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complexity of the pre-treatment significantly reduces the detection throughput and easily causes contamination of the samples to be tested.

Method used

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Examples

Experimental program
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Embodiment 1

[0019] Example 1, an integrated centrifuge according to the present invention is applied to the colloidal gold method detection after dairy protein precipitation

[0020] Operation steps: ①Add dairy products to the rotor and add reaction reagent A; ②Put capsule reagent B in the slot of the rotor; ③Turn on the motor power; ④After the rotor is covered with a composite stirrer, then composite stirring at speed 1 (low speed, about 600rpm) Mix the reagent A with the dairy product to be tested, and the first chemical reaction occurs to produce protein precipitation; ⑤ Switch to speed 2 (high speed, about 3000rpm), and quickly realize the aggregation and separation of denatured proteins at the bottom of the grid with the help of the grid ; ⑥ Switch to speed 3 (medium speed, about 1500rpm), the capsule reagent B in the card slot is dissolved and released, and the second chemical reaction is carried out; ⑦ Repeat step 5 to separate the newly produced protein precipitate; ⑧ Stop the cent...

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Abstract

The invention discloses an integrated centrifuge. The integrated centrifuge aims at achieving the purpose of integrating uniform mixing, centrifuging and chemical reactions in dairy product treatmentin one device, the sample pretreatment time is shortened, the pretreatment efficiency of to-be-detected dairy products is improved, and the the sensitivity and the detection flux of measuring chemicalcontaminants in dairy products through an existing colloidal gold immunodetection product are improved. The integrated centrifuge comprises a composite stirrer, a rotor and a motor, and integrates the steps of uniform mixing, centrifugalization, chemical reactions and the like. The composite stirrer is a device with the effect of a top cover and the effect of stirring. The rotor is a device whichis of an inverted-cone-shaped structure and is used for containing liquid and achieving multiple chemical reactions and physical separation. The motor is a device for providing power for centrifugalization. Uniform mixing, centrifugalization, medicine release and chemical reactions can be achieved at different rotating speeds of the rotor. The integrated centrifuge can be used for treating materials such as the dairy products, blood and tissue, can be used for sample pretreatment in multiple fields such as clinical diagnosis, inspection and quarantine, food safety inspection, judicial expertise and poison detection, and has broad market prospects.

Description

technical field [0001] The invention relates to a chemical reaction device, in particular to a centrifuge which integrates mixing, centrifugation and chemical reaction operations, and belongs to the technical field of biomedicine. Background technique [0002] Sample handling is essential in various inspection and testing practices. Especially for samples with complex matrices, different processing methods are set up for different detection instruments. Mixing and centrifugation steps accompanied by certain chemical reactions are the most common operations. Among them, mixing is mainly carried out on vortex and oscillation equipment to achieve mixing of different components or specific reactions; centrifugation mainly relies on different types of centrifuges and supporting rotors to achieve separation of different components; chemical reactions mainly Relying on a variety of special equipment. [0003] Take the colloidal gold test card to detect chemical pollutants in dai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B04B5/12G01N1/28G01N1/38G01N33/53
Inventor 王升启郄志伟肖瑞
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA