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Manganese-resistant Providence bacterial strain and method for repairing heavy metal-contaminated soil

A technology that pollutes soil and heavy metals, applied in the field of microorganisms, can solve the problems of unrepaired heavy metal manganese-contaminated soil, etc., and achieve the effect of not destroying soil fertility, reducing accumulation, and good repairing effect

Active Publication Date: 2021-02-12
HUNAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the research on Providencia at home and abroad is related to medicine, and there is no report on the restoration of heavy metal manganese-contaminated soil.

Method used

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  • Manganese-resistant Providence bacterial strain and method for repairing heavy metal-contaminated soil
  • Manganese-resistant Providence bacterial strain and method for repairing heavy metal-contaminated soil
  • Manganese-resistant Providence bacterial strain and method for repairing heavy metal-contaminated soil

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Embodiment Construction

[0020] The manganese-resistant Providence bacterial strain screening and application process of the present invention are as follows:

[0021] Step 1, isolate the strain

[0022] Soil sampling and treatment: Use the multi-point random sampling method to collect heavy metal-contaminated soil samples 5-20 cm underground from an industrial park in Qingshuitang, Zhuzhou City, and mix them. Mix the sample with sterile water at a ratio of 1:9 and let it stand. Take 2 mL of the supernatant and add it to the solution containing Mn at a concentration of 50 mM 2+ 、Cd 2+ , Pb 2+ 、Cu 2+ 、Cr 6+ , Zn 2+ 100mL liquid LB medium, and then placed in a constant temperature shaker incubator at 35°C for about 48h of cultivation.

[0023] Step 2, bacterial purification

[0024] Take 1mL of the bacterial solution in step 1, apply the gradient dilution plate method, evenly spread on the LB solid medium, and invert for 12-24h, it is found that only Mn 2+ Bacteria grew on solid medium. Pick a...

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Abstract

The invention discloses a bacterial strain of the genus Providence resistant to manganese and a method for repairing heavy metal polluted soil. The preservation number of the strain is CCTCC M 2018876, named Providencia sp.LLDRA6. The method of the invention can effectively absorb heavy metals in soil, thereby reducing the accumulation of heavy metals in soil. The method has the advantages of low cost, good restoration effect, no damage to soil fertility, and no secondary pollution of soil.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular, the invention relates to a manganese-resistant Providence bacterial strain and a method for remediating heavy metal polluted soil. Background technique [0002] In the entire terrestrial ecosystem, soil is the most important component. It is not only an irreplaceable environmental factor for humans and animals to live in, but also a basic guarantee for food safety and human health. And soil plays an important role in protecting the environment and maintaining ecological balance. With the rapid development of industrialization, urbanization and modern agriculture, heavy metals have been more and more used in industry and agriculture. Moreover, due to backward technology and irrational use of resources, more and more heavy metals have entered water bodies, soils and other media in various ways and forms, which has affected the quality of drinking water, cultivated land, agricultural p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
Inventor 李丁阮小芳李如意丁哲旭陈莎汤建新曾晓希
Owner HUNAN UNIV OF TECH