Blood sampler for preparing bovine tuberculosis stimulation supernatant

Abstract

The invention firstly provides a blood sampler for preparing bovine tuberculosis stimulation supernatant. The blood sampler comprises an inner cylinder and other components commonly used by the bloodsampler; a mixture of heparin and a cocktail antigen is placed in the inner cylinder of the blood sampler; the cocktail antigen comprises ESAT6, CFP10, FixB and Rv3515c protein. According to the bloodsampler, a traditional method is simplified, and detection can be directly carried out after blood collection and incubation. The whole testing process can be carried out in a common laboratory, andfour hours can be saved when each person detects the simulation supernatant of 50 cattle. An original complex bovine tuberculosis gamma-interferon test becomes very easy to popularize. Meanwhile, thecocktail antigen used in the blood sampler can eliminate false positive reactions caused by vaccine immunity.

Description

technical field [0001] The invention belongs to the field of animal epidemic detection equipment, and in particular relates to a blood sampling device for preparing supernatant stimulated by bovine tuberculosis. Background technique [0002] Bovine tuberculosis is a chronic consumptive zoonotic disease. It is an animal disease that must be notified according to the International Organization for Animal Health (OIE). It is a second-class animal infectious disease in my country. pose a major threat. According to statistics, about 5% of human tuberculosis is caused by Mycobacterium bovis. Therefore, it is of great public health significance to grasp the current situation of bovine tuberculosis in my country and to eliminate infected cattle through quarantine. The quarantine of bovine tuberculosis can be classified into three categories at present, bacteriological detection, molecular biological detection and immunological detection. The sample collection of bacteriological de...

AI Technical Summary

Problems solved by technology

[0010] It can be seen that the preparation process of the stimulated supernatant used in the bovine tuberculosis gamma interferon ELISA test is cumbersome, the amount of reagent consumables is large, and the workload is heavy. In addition to the time to harvest the supernatant, each person can prepare the stimulated supernatant of 50 cattle. takes 5 or more hours

At the same time, the determination of ELISA results is relatively complicated, and it is difficult to promote it in laboratories with poor conditions, and it cannot rule out false positive reactions caused by vaccine immunization.

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