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TLC identification method of Pseudogentian acuminate

A technique of Pseudomonas acuminate and thin-layer chromatography, which is applied in the field of pharmaceutical analysis, can solve problems such as the thin-layer identification method of Pseudomonas acuminate, and achieve high identification sensitivity, good separation effect, and high identification accuracy Effect

Active Publication Date: 2021-12-10
HEBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the thin-layer identification method has many advantages such as high separation effect, fast identification, and high sensitivity, it has been widely used in the production process of Chinese medicinal materials, and the TLC identification method of commonly used Chinese medicinal materials is disclosed in the "Chinese Pharmacopoeia". However, because Pseudomonas acuminate belongs to Mongolian medicine, it has not yet entered the "Chinese Pharmacopoeia", so there has not been any relevant report on the TLC identification method of Pseudomonas acuminatum

Method used

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  • TLC identification method of Pseudogentian acuminate
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  • TLC identification method of Pseudogentian acuminate

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Experimental program
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Effect test

Embodiment 1

[0039] The identification method of the present embodiment includes the following steps:

[0040] (1) Get 2.0g of leaf flower, root and whole plant powder of Pseudomonas acupoints respectively, add 50ml of methanol solution, weigh, under the process conditions of power 250W, frequency 50HZ, ultrasonic extraction 23min, weigh again, and Make up the reduced weight with methanol solution, shake well, and filter to obtain the test solution I, II, III;

[0041] (2) Get the daisy gentianone reference substance and the demethyl daisy gentianone reference substance, and add methanol solution to configure respectively, so as to obtain the daisy gentianone reference substance solution with a concentration of 0.5 mg / ml, and the concentration is 0.5 mg / ml of demethyl daisy leaf gentianone reference substance solution;

[0042] (3) Thin-layer chromatography test: draw 5 μl each of the test solution I, II, and III, and 5 μl each of the two reference substance solutions, and spot on the sam...

Embodiment 2

[0045]The identification method of the present embodiment includes the following steps:

[0046] (1) Get 2.0g of leaf flower, root and whole plant powder of Pseudomonas acupoints respectively, add 40ml of methanol solution, weigh, under the process conditions of power 250W, frequency 50HZ, ultrasonic extraction 30min, weigh again, and Make up the reduced weight with methanol solution, shake well, and filter to obtain the test solution I, II, III;

[0047] (2) Get the daisy gentianone reference substance and the demethyl daisy gentianone reference substance, and add methanol solution to configure respectively, so as to obtain the daisy gentianone reference substance solution with a concentration of 0.5 mg / ml, and the concentration is 0.5 mg / ml of demethyl daisy leaf gentianone reference substance solution;

[0048] (3) Thin-layer chromatography test: draw 5 μl each of the test solution I, II, and III, and 5 μl each of the two reference substance solutions, and spot on the same...

Embodiment 3

[0051] The identification method of the present embodiment includes the following steps:

[0052] (1) Get 2.0g of leaf flower, root and whole plant powder of Pseudomonas acupoints respectively, add 30ml of methanol solution, weigh, under the process conditions of power 250W, frequency 50HZ, ultrasonic extraction 20min, weigh again, and Make up the reduced weight with methanol solution, shake well, and filter to obtain the test solution I, II, III;

[0053] (2) Get the daisy gentianone reference substance and the demethyl daisy gentianone reference substance, and add methanol solution to configure respectively, so as to obtain the daisy gentianone reference substance solution with a concentration of 0.6mg / ml, and the concentration is 0.6mg / ml of demethyl daisy leaf gentianone reference substance solution;

[0054] (3) Thin-layer chromatography test: draw 5 μl each of the test solution I, II, and III, and 5 μl each of the two reference substance solutions, and spot on the same ...

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Abstract

The present invention relates to a TLC identification method of Pseudomonas acuminate, to identify whether the medicinal material of Pseudomonas acuminate is up to standard, the identification method comprises preparation of test solution, preparation of reference solution, TLC identification test, and The chromatogram of the test product is compared with the chromatogram of the daisy leaf gentianone reference substance and the daisy leaf gentianone reference substance chromatogram respectively, and when the spots of the same color are displayed at the corresponding positions, the said pseudogentiana acupoints medicinal material is Qualified product. The thin-layer chromatography identification method of Pseudomonas acuminatee of the present invention, by distinguishing whether to contain the important component daisy leaf gentianone and demethyl daisy phyllogentiane in the test sample, thereby can quickly To identify whether the quality of the sample is qualified or not, this identification method not only has the advantages of small sample amount, good separation effect, high identification sensitivity, and high identification accuracy, but also fills the gap in the application of TLC identification technology for Pseudomonas acuminate.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a thin-layer chromatographic identification method of Pseudomonas acuminate. Background technique [0002] Pseudomonas acuminates is derived from the genus Pseudomonas acuminates of Gentianaceae, which are mostly distributed in Mongolia, the Soviet Union, North America, Hebei, the eastern part of the Mongolian Plateau, Northeast my country, Ningxia and other regions in China. Pseudomonas acupoints is mostly used in Mongolian and Tibetan medicine, and generally the whole herb is used as medicine. Mongolian medicine calls it "Agut-Qiqige", and Tibetan medicine calls it "Sangdige". The main production areas are Keshiketeng Banner, Xilin Gol League, Xiwuzhumuqin Banner, Ulanchabu League, Bayannaoer League, and Alxa Left Banner of Alxa League in Chifeng City. [0003] At present, with the gradual research on the medicinal material of pseudogentiana pointed leaf, it is found tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 李爱英郭慧马东来温子帅
Owner HEBEI UNIV OF CHINESE MEDICINE
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