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A kind of fluorescent probe and its preparation method and application

A technology of fluorescent probes and compounds, applied in the field of biochemical analysis, can solve the problems of oxidative interference and biological toxicity, and achieve the effect of high reactivity and short lifespan

Active Publication Date: 2020-07-07
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these probes are often oxidatively interfered by other reactive oxygen species (such as hydrogen peroxide, hypochlorous acid, peroxynitroso), or require the addition of dimethyl sulfoxide (DMSO), which is toxic to organisms.

Method used

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  • A kind of fluorescent probe and its preparation method and application
  • A kind of fluorescent probe and its preparation method and application
  • A kind of fluorescent probe and its preparation method and application

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Embodiment Construction

[0037] Hereinafter, exemplary embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. Although the drawings show exemplary embodiments of the present disclosure, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0038] Example 1 Preparation of fluorescent probe shown in formula II

[0039] This embodiment provides a preparation method of the fluorescent probe represented by formula II, and its synthetic route is as follows:

[0040]

[0041] Specific steps are as follows:

[0042] The compound represented by formula V (562 mg, 2 mmol), the compound represented by formula VI (164 mg, 1 mmol) and sodium acetate (100 mg) were disso...

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Abstract

The invention relates to a dual-function fluorescent probe for detecting hydroxyl radicals and viscosity and a preparation method and application of the fluorescent probe. The structure of the fluorescent probe is shown in the formula I, n is an integer from 1-6, and R is alkyl of C1-6. According to the dual-function fluorescent probe, the hydroxyl radicals and the viscosity can be simultaneouslydetected with high sensitivity and selectivity in two independent analysis wavelength ranges. In addition, the dual-function fluorescent probe is stable in property, the preparation method is easy andconvenient to implement, large-batch production can be achieved, and the fluorescent probe has high application prospects in the fields such as food, environment and biology. The formula I is shown in the description.

Description

Technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a fluorescent probe and a preparation method and application thereof. Background technique [0002] Hydroxyl free radical is a reactive oxygen species with extremely high reactivity. In cells, it can damage important biological molecules such as protein, DNA, and lipids, thereby affecting the normal physiological functions of cells and causing a series of diseases, such as arteriosclerosis, Neurodegenerative diseases, cancer, etc. Hydroxyl radicals have the characteristics of high reactivity, short life (nanosecond level), and short diffusion distance (nano-level). Therefore, the analytical sensitivity and selectivity of hydroxyl radical probes are extremely high during practical application. The current hydroxyl radical probes on the market mainly include: APF, HPF, CM-H based on oxidation reaction 2 DCFDA, etc.; Proxyl fluorescamine, TEMPO-9-AC, etc. based on free rad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/10C09K11/06G01N21/64
Inventor 马会民李洪玉李晓花史文
Owner INST OF CHEM CHINESE ACAD OF SCI