Method for regulating and controlling accumulation of spirulina nutrient element zinc element
A nutrient element and spirulina technology, which is applied in the field of regulating the accumulation of spirulina nutrient element zinc to achieve the effects of increasing the content, improving the absorption and utilization rate of the human body, and reducing irritation.
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Embodiment 1
[0034] 1) Inoculate Spirulina into Z's medium, after inoculating Spirulina, the OD of algae liquid 560 The value is 0.3. Cultivate for 3 days at 30°C, 12klx, pH=9.5.
[0035] 2) Continue culturing the spirulina cultured in step 1) for 3 days, adjust the temperature to 20°C, control the light at 12klx, and control the pH at 8.5, and add exogenous calcium CaCO to the medium 3 To a final concentration of 0.6g / L, cultivated for 3 days to obtain calcium-rich spirulina (calcium element content in spirulina is 2.52mg / g). The organic degree of calcium is 53.57%. Spirulina growth rate K=0.0465.
Embodiment 2
[0037] 1) Inoculate Spirulina into Z's medium, after inoculating Spirulina, the OD of algae liquid 560 The value is 0.3. Cultivate for 3 days at 30°C, 12klx, pH=9.5.
[0038] 2) Continue culturing the spirulina cultured in step 1) for 3 days, adjust the temperature to 20°C, control the light at 12klx, and control the pH at 8.5, and add exogenous calcium CaCO to the medium 3 To a final concentration of 0.6g / L, cultivate for 5 days to obtain calcium-rich spirulina (calcium element content in spirulina is 5.64mg / g). The organic degree of calcium is 72.14%. Spirulina growth rate K=0.0448.
Embodiment 3
[0040] 1) Inoculate Spirulina into Z's medium, after inoculating Spirulina, the OD of algae liquid 560 The value is 0.3. Cultivate for 3 days at 30°C, 12klx, pH=9.5.
[0041] 2) Continue culturing the spirulina cultured in step 1) for 3 days, adjust the temperature to 20°C, control the light at 12klx, and control the pH at 8.5, and add exogenous calcium CaCO to the medium 3 To a final concentration of 0.6g / L, cultivated for 7 days to obtain calcium-rich spirulina (calcium element content in spirulina is 18.21mg / g). The organic degree of calcium is 84.17%. Spirulina growth rate K=0.0411.
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