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Microbial agent composition for facilitating Macadamia nut growth

A microbial agent and composition technology, applied in the direction of microorganism-based methods, microorganisms, biocides, etc., can solve the problems of short plants, affecting the absorption and utilization of water and nutrients, and plant deformities

Pending Publication Date: 2019-06-07
JIANGCHENG ZHONGAO AGRI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment method of this patent has the following disadvantages: as a chemically synthesized plant growth regulator, paclobutrazol, long-term use will lead to short plant growth, plant deformity, etc.; konjac glucomannan has gelling and film-forming properties, and konjac glucomannan Glycans become gelatinous when exposed to water. If konjac glucomannan dissolves in water and covers the surface of the root system of macadamia nuts, it will affect the absorption and utilization of water and nutrients by the roots

Method used

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  • Microbial agent composition for facilitating Macadamia nut growth
  • Microbial agent composition for facilitating Macadamia nut growth
  • Microbial agent composition for facilitating Macadamia nut growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: A preparation example of a microbial bacterial composition that is beneficial to the growth of macadamia nuts

[0048] (1) Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus and Bacillus coliformis were respectively expanded and fermented with nutrient gravy liquid medium to obtain 5 kinds of fermentation broths. The preparation method of the nutrient gravy liquid medium is as follows: take 10.0 g of peptone, 3.0 g of beef extract, 5.0 g of sodium chloride and 1.0 L of distilled water, mix and dissolve, and adjust the pH of the medium to pH 7.0. The culture process adopts the bacterial shaker to shake the bacteria, cultures at 25° C. for 3 days, and obtains the Bacillus bacterial liquid of 5 kinds of bacteria in the exponential growth phase.

[0049] (2) Get the bacterium liquid 8000rpm that obtains in step (1) and centrifuge 20min, get respectively 5 kinds of thalline precipitations, with Bacillus licheniformis, Bacillus megateriu...

Embodiment 2

[0061] Example 2: A preparation example of a microbial bacterial agent composition that is beneficial to the growth of macadamia nuts

[0062] (1) Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus and Bacillus colioids were respectively cultured and fermented with plasma substituting liquid medium to obtain 5 kinds of fermentation broths. The preparation method of the plasma substitute liquid medium is as follows: take 2.0 g of peptone, 130 g of sucrose, 0.3 g of potassium dihydrogen phosphate, 1.0 g of disodium hydrogen phosphate and 1.0 L of distilled water, mix and dissolve, and adjust the pH of the medium to pH 7.2. The culture process adopts the bacterial shaker to shake the bacteria, cultures at 25° C. for 3 days, and obtains the Bacillus bacterial liquid of 5 kinds of bacteria in the exponential growth phase.

[0063] (2) Get the bacterium liquid 12000rpm that obtains in the step (1) and centrifuge 10min, get respectively 5 kinds of thalline...

Embodiment 3

[0076] Example 3: A preparation example of a microbial bacterial agent composition that is beneficial to the growth of macadamia nuts

[0077] (1) Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus and Bacillus coliformis were respectively expanded and fermented with nutrient gravy liquid medium to obtain 5 kinds of fermentation broths. The preparation method of the nutrient gravy liquid medium is as follows: take 10.0 g of peptone, 3.0 g of beef extract, 5.0 g of sodium chloride and 1.0 L of distilled water, mix and dissolve, and adjust the pH of the medium to pH 7.0. The culture process adopts the bacterial shaker to shake the bacteria, cultures at 25° C. for 3 days, and obtains the Bacillus bacterial liquid of 5 kinds of bacteria in the exponential growth phase.

[0078](2) Get the bacterium liquid 10000rpm that obtains in step (1) and centrifuge 15min, get respectively 5 kinds of thalline precipitations, with Bacillus licheniformis, Bacillus meg...

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Abstract

The invention belongs to the field of plant cultivation and relates to a microbial agent composition, in particular to a microbial agent composition for facilitating Macadamia nut growth. The composition comprises raw bacterial strains in parts by weight as follows: 2-3 parts of bacillus licheniformis, 1-2 parts of bacillus megaterium, 1-2 parts of bacillus pumilus, 2-3 parts of bacillus cereus and 3-5 parts of bacillus mucilaginosus. The composition can induce tree roots to grow deeper into the soil, and has effects of improving soil microecological environment, alleviating diseases and strengthening nutrition. The microbial agent composition can be applied to practices, such as Macadamia tree cultivation and growing and the like.

Description

technical field [0001] The invention belongs to the field of plant planting, and relates to a combination of microbial agents, in particular to a composition of microbial agents beneficial to the growth of macadamia nuts. Background technique [0002] Macadamia integrifolia F.Muell, also known as macadamia nut, is native to Australia, and its history of artificial cultivation is only more than 150 years. It is a new kind of fruit tree. Macadamia fruit trees, especially the younger ones, have poor fruit setting rate and severe flower and fruit drop, which affect the early yield. The main reason is that the main roots of macadamia fruit trees are underdeveloped, and the main root systems are distributed in the shallow layer of the soil. During the dry spring flowering period, fruit trees cannot obtain water and nutrients from deep soil for young fruit to grow. In addition, the root system distribution of macadamia fruit trees also makes the fruit trees weak against wind, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00A01P21/00C12R1/10C12R1/11C12R1/085C12R1/07
Inventor 徐斌王怡施蕊李彪
Owner JIANGCHENG ZHONGAO AGRI TECH DEV
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