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Molecular marker for detecting fusarium head blight (FHB)-resistant QTL Qfhb.hbaas-5AL and use method
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A technology for scab and wheat scab, which is applied in the field of molecular markers for detecting scab-resistance QTLQfhb.hbaas-5AL, and can solve the problem of less application of gene breeding and the like
Active Publication Date: 2019-06-07
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI +1
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However, except for Fhb1, the breeding applications of other genes are less reported
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Embodiment 1
[0046] Example 1, Discovery of Qfhb.hbaas-5AL and its associated SNP markers
[0047] Test materials: WAPS (Wheat Association Panel for Scab research) population formed from 240 domestic and foreign wheat varieties (lines), see Table 1. The materials used are recorded in the literature: Zhu Zhanwang, Xu Dengan, Cheng Shunhe, et al. Identification and traceability of the stripe rust resistance gene Fhb1 in Chinese wheat varieties [J]. Acta Crops, 2018, 44(4): 473–482. The public can download from Hubei Obtained from Food Crops Research Institute of Provincial Academy of Agricultural Sciences.
[0048] Table 1 Varieties (lines) and their sources of WAPS population
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[0058] a 90K SNP chiptyping results; b Indicates the detection result of the marker M-5AL-Fhb; "-" indicates that the chip data is missing.
[0059] 1. Discovery of Qfhb.hbaas-5AL and its associated SNP mark...
Embodiment 2
[0102] Embodiment 2, actual sample detection
[0103] Test materials: WAPS species shown in Table 1.
[0104] 1. Identification of scab resistance
[0105] With embodiment 1.
[0106] 2. M-5AL-Fhb marker detection
[0107] Mark with M-5AL-Fhb according to the method in embodiment 1, carry out PCR amplification to 240 kinds shown in table 1, then digest PCR product with Age I endonuclease, detect restriction product;
[0108] If the digested product is only 334bp in size, the genotype of SNPIWB42293 is CC; if the digested product is 213bp and 121bp fragments, the genotype of SNPIWB42293 is TT.
[0109] The SNP site IWB42293 genotype is CC, and the scab resistance of the tested wheat is higher or candidate higher than that of the IWB42293 genotype, and the tested wheat is TT.
[0110] The marker detection results of 240 wheat varieties and the average value of FHB index are shown in Table 1. The results in Table 1 were statistically analyzed to obtain the results in Table 4...
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Abstract
The invention discloses a molecular marker for detecting fusariumhead blight (FHB)-resistant QTL Qfhb.hbaas-5AL and a use method. The invention provides an application of a substance for detecting the genotype of an SNP IWB42293 locus of wheat chromosome 5AL in identification or auxiliary identification of resistance of wheat FHB and also provides an application of the substance for detecting thegenotype of the SNP IWB42293 locus of the wheat chromosome 5AL in preparation of a product for identification or auxiliary identification of resistance of the wheat FHB. The anti-FHB locus Qfhb.hbaas-5AL located on a short arm of the wheat 5A chromosome by genome wide association study (GWAS), associated SNP IWB42293 is further converted into common PCR marked M-5AL-Fhb, and the marker can be used for detecting the genotype of the FHB-resistant QTL Qfhb.hbaas-5AL and applied to anti-FHB molecular breeding.
Description
technical field [0001] The invention relates to the field of biological technology, in particular to a molecular marker for detecting scab resistance QTL Qfhb.hbaas-5AL and a method for using it. Background technique [0002] Fusariumhead blight (FHB) caused by Fusarium graminearum is a widespread fungal disease that seriously affects wheat yield and quality. More importantly, the susceptible grains contain mycotoxins, such as deoxynivalenol (DON), which endanger the health of humans and animals and affect food and feeding. [0003] The middle and lower reaches of the Yangtze River and the Northeast wheat region in my country are areas where wheat scab frequently occurs and reoccurs. In recent years, affected by global climate change and the popularity of straw returning under the wheat-corn rotation system, the disease has shown a trend of expansion and aggravation, and has become a common disease in the Huanghuai wheat region. At present, the average annual occurrence a...
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