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Method for identifying fruit storage resistance of apple plants and specific primer pair used thereby

A specific primer pair and storage-resistant technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of difficult breeding, high cost, and long cycle, and achieve major Application value, reduction of breeding cost, effect of improving breeding efficiency

Active Publication Date: 2019-06-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Apple is a complex polyploid tree species, and the offspring of hybrids will segregate widely. Breeding is difficult, costly, and long-term. Especially when multiple quality traits are integrated, the segregation of offspring traits is more diverse and more difficult.

Method used

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  • Method for identifying fruit storage resistance of apple plants and specific primer pair used thereby
  • Method for identifying fruit storage resistance of apple plants and specific primer pair used thereby

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Experimental program
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Embodiment

[0048] One, the establishment of the method for identifying the fruit storage resistance of apple plants

[0049]The inventors of the present invention have established a method for identifying the fruit storage resistance of apple plants through a large number of experiments. details as follows:

[0050] With the genomic DNA of the apple to be tested (the pulp of the fruit or the leaf of the plant) as a template, primer F: 5'-AGAGAGATGCCATTTTTGTTCGTAC-3' (sequence 1 in the sequence listing) and primer R: 5'-CCTACAAACTTGCGTGGGGATTATAAGTGT-3' (sequence The primer pair composed of sequence 2) in the list is subjected to PCR amplification to obtain a PCR amplification product. If the PCR amplification product is one and the size is 655bp, the genotype of the apple plant to be tested is ACS1-2 / -2 homozygous type; if the PCR amplification product is two kinds and the size is 517bp and 655bp respectively, then the genotype of the apple plant to be tested is ACS1-1 / -2 heterozygous t...

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Abstract

The invention discloses a method for identifying fruit storage resistance of apple plants and a specific primer pair used thereby. The specific primer pair is composed of a primer F and a primer R; the primer F is a single-stranded DNA molecule shown in Sequence 1 of the sequence listing; and the primer R is a single-stranded DNA molecule shown in Sequence 2 of the sequence listing. Tests have proven that the specific primer pair is capable of identifying genotypes of apple plants ACS1 when the apple plants ACS1 are still at seedling stage; and then, fruit storage resistance of the apple plants ACS1 can be determined, thereby helping to save land, manpower and material costs. The method for identifying the fruit storage resistance of the apple plants is of great significance for improvingbreeding efficiency and saving breeding cost, and has great application values in apple breeding.

Description

technical field [0001] The invention belongs to the field of plant molecular breeding, and in particular relates to a method for identifying the fruit storage resistance of apple plants and a pair of specific primers used therefor. Background technique [0002] The apple industry plays a vital role in increasing farmers' income and rural development. At present, the variety structure of my country's apple industry is not reasonable. 'Red Fuji' accounts for more than 70% of my country's apple production. Refined varieties are updated. Excellent fresh apple varieties should have good appearance, crisp and juicy, full of flavor and storage resistance. [0003] Apple is a complex polyploid tree species, and the traits of hybrid progeny will segregate extensively. Breeding of varieties is difficult, costly, and long-term. Especially when multiple quality traits are integrated, the traits of offspring are more diverse and difficult to segregate. At present, the use of molecular ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q1/6827
Inventor 张宗营王意程王翠翠毛作霖王楠苏梦雨张静姜生辉陈学森
Owner SHANDONG AGRICULTURAL UNIVERSITY