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Human oral mucosa model and preparation method thereof

A technology of oral mucosa and human body, which is applied in the field of human oral mucosa model and its preparation, can solve the problems that cannot reflect the structure of natural oral mucosa well

Inactive Publication Date: 2019-06-14
广州舒客实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the structure constructed by the prior art model cannot well reflect the structure of the human natural oral mucosa.

Method used

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  • Human oral mucosa model and preparation method thereof
  • Human oral mucosa model and preparation method thereof
  • Human oral mucosa model and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0036] A human oral mucosa model comprises a bottom-up arrangement of a basal layer, a lamina propria and an epithelial layer, the lamina propria includes oral fibroblasts and biological scaffold materials, and the epithelial layer includes oral epithelial cells.

[0037] The base layer includes a supporting membrane, and the material used for the supporting membrane may be a nylon membrane, a silicone membrane, a rubber membrane or a synthetic polymer material membrane. For example, the supporting film used is a synthetic polymer film, a PE film or a PET film.

[0038] The source of the oral fibroblasts is not limited by the present invention, and may be stem cells capable of differentiating into fibroblasts, primary cells isolated and cultured from oral tissue, or commercial immortalized oral fibroblasts.

[0039] The source of the biological scaffold material is not limited by the present invention, it can be collagen, extracellular matrix complex, hyaluronic acid, polysacc...

Embodiment 2

[0044] A preparation method of a human oral mucosa model, the preparation process comprising:

[0045] Step 1. Expansion and culture of oral keratinocytes:

[0046] Oral keratinocytes were collected, recovered and expanded, and P5 cells were used, digested with trypsin to make a single cell suspension, and the cell density was adjusted to 5.0×10 5 Cells / mL, inoculate in a culture dish, add culture solution Ⅰ for culture, shake the culture bottle gently to make the cells evenly dispersed, and place at 37°C, 5% CO 2 cultivated under conditions;

[0047] The culture medium I includes the following components:

[0048]

[0049] Step 2, expansion and culture of gingival fibroblasts:

[0050] Oral keratinocytes were collected, recovered and expanded, and P5 cells were used, digested with trypsin to make a single cell suspension, and the cell density was adjusted to 5.0×10 5 cells / mL, inoculate in a culture dish, add culture medium II for culture, shake the culture bottle gently...

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Abstract

The invention belongs to the technical field of tissue engineering and particularly relates to a human oral mucosa model and a preparation method thereof. The mucosa model comprises a basal layer anda lamina propria, also comprise an epithelial layer and accordingly is highly approximate to the epithelial tissue structure of the human oral mucosa. The model can be applied to safety and efficacy detection of oral care products, small molecular compounds, active proteins, medical devices, chemicals, disposable sanitary products and other products and safety and efficacy evaluation of other materials in contact with the oral mucosa, and the obtained detection data and evaluation results are closer to the evaluation results of the oral mucosa.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a human oral mucosa model and a preparation method thereof. Background technique [0002] In March 2009, EU Cosmetics Regulation (EC) 1223 / 2009 was officially implemented, and animal experiments on cosmetics and chemicals have been banned. The traditional 2D model can only be used to study cell migration and proliferation, but cannot be used for oral mucosal function, drug percutaneous absorption and pathological research. Compared with the 3D model, the cell behavior of the 2D model is significantly different, and the effective raw materials or products screened under the 2D culture model are not ideal for clinical efficacy. However, oral mucosal tissue derived from human body mainly has difficulties in obtaining materials and limited sources, which cannot meet the quantity requirements for in vitro evaluation of oral cavity-related products. [0003] Eu...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/077
Inventor 陈敏珊李平李林王真史
Owner 广州舒客实业有限公司
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