Compound enzyme for preparing pulp, and preparation method and use method of compound enzyme

A composite enzyme and pulp technology, applied in textile and papermaking, fiber raw material processing and other directions, can solve the problems of difficult preservation of biological enzymes, unfavorable slurry purification, difficulty in effect control, etc., to improve the pretreatment effect, prolong the storage time, and produce low cost effect

Active Publication Date: 2019-06-14
秾源科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological enzymes used in the pretreatment step of biopulping are cellulase, hemicellulase, lignin degrading enzyme and pectinase. Will choose to directly use industrially produced biological enzymes for production, but the direct use of industrially produced biological enzymes has difficulties in recovering the enzymes used, huge usage, increased production waste, and is not conducive to further purification of the slurry. At the same time, there are enzymes in the biological enzyme treatment. Low efficiency, difficult effect control, difficult storage of biological enzymes, and high requirements for use

Method used

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  • Compound enzyme for preparing pulp, and preparation method and use method of compound enzyme
  • Compound enzyme for preparing pulp, and preparation method and use method of compound enzyme
  • Compound enzyme for preparing pulp, and preparation method and use method of compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0053] This experiment example is in the present invention, the determination experiment of the enzymatic activity of the enzymatic activity of the obtained compound solution to the immobilization of cellulase, hemicellulase, lignin degrading enzyme and pectinase by the enzyme carrier prepared in the invention by the applicant .

[0054] Experimental materials: 10mg / mL enzyme carrier solution, 10mg / mL cellulase solution, 10mg / mL hemicellulase solution, 10mg / mL lignin degrading enzyme solution, 10mg / mL pectinase solution, Ionized water, pH=6 phosphate buffer.

[0055] making process:

[0056] 1. Take 10g of enzyme carrier solution, put it into the reaction kettle, add 30g of pH=6 phosphate buffer solution, 100g of raw material enzyme solution, place at 5°C for 24h, take it out and wash it with phosphate buffer solution to obtain a composite solution;

[0057] 2. Shake the compound enzyme solution obtained in step 1 at room temperature for 20 minutes, centrifuge for 5 minutes,...

experiment example 2

[0070] This experimental example is an experiment in which the applicant compares the effect of pulp pretreatment using pulping compound enzymes and other prior art pulp pretreatment effects in the present invention.

[0071] The pulp raw material used in this practical example is cotton stalk.

[0072] Preparation process a:

[0073] A kind of pulp is prepared by adopting the pretreatment process of the Chinese patent application (CN201710358393.7) in the prior art.

[0074] S1: Cut the cotton stalks into cotton stalk pieces, and transport them to the primary pretreatment immersion solution A. The primary pretreatment immersion solution A, in parts by weight, includes the following raw materials: 6 parts of alkylphenol polyoxyethylene ether, 0.5 parts of triethanolamine, 0.7 parts of stearic acid, 1 part of oleic acid, 1.2 parts of lauric acid, and 60 parts of water were soaked for 45 minutes under the action of ultrasonic waves, and then filtered to obtain the steeping mate...

experiment example 3

[0097] This experimental example is an experiment conducted by the applicant on the immobilization effect of hemicellulase and pectinase by using graphene oxide as a carrier in the present invention.

[0098] Experimental process A:

[0099] S1: Take 20kg of wood chips and place them in a sealed device, add a compound enzyme solution, the ratio of hemicellulase to pectinase in the graphene oxide compound enzyme solution is 5:1, and add deionized water to combine the enzyme solution with The slurry is mixed evenly, the dosage of hemicellulase is 5U / g absolute dry wood chips, and the consumption of pectinase is 1U / g absolute dry wood chips.

[0100] S2: react the solution obtained in step S1 at 45° C. for 1 hour, and slowly stir the material so that the material and the enzyme solution are fully mixed.

[0101] S3: heat the solution obtained in step S2 for 15 hours, and extract the prepared slurry.

[0102] S4: measuring the enzyme activity of the remaining reaction solution. ...

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Abstract

The invention provides a compound enzyme for preparing pulp. The compound enzyme comprises an enzyme carrier and a bio-enzyme, the enzyme carrier is graphene oxide, the bio-enzyme is one or more of cellulase, hemicellulase, ligninase and pectinase, and the bio-enzyme is fixed to the enzyme carrier in an adhering mode. By adopting a method, the bio-enzyme can be effectively fixed to the graphene oxide carrier, the high-proportion surface area and good biocompatibility of the graphene oxide are fully utilized, functional groups rich on the surface of the graphene oxide also have the acidic chemical property, and the action of neutralizing alkaline ions in a solution can be achieved, so that the pH value of the local part around the graphene oxide is decreased, thus the bio-enzyme adhering to the surface of the graphene oxide exerts activity advantageously, the bio-enzyme is fixed by the graphene oxide, most of the bio-enzyme remains in a liquid environment so as to be secondarily utilized, the utilization efficiency of the bio-enzyme is improved, and production waste is reduced.

Description

Technical field: [0001] The invention relates to the fields of pulping and papermaking and utilization of biomass resources, in particular to a composite enzyme for pulping and its preparation method and use method. Background technique: [0002] Lignocellulosic raw material, as an important and abundant biomass resource in nature, is an important production raw material in the current paper industry. In the prior art, the process of using microorganisms themselves or enzymes produced by microorganisms to pretreat fiber raw materials and then combine with corresponding pulping processes to produce pulp is called biopulping. For biopulping, its pretreatment step is the most important, because the use of bioenzyme pretreatment has little damage to the fibers in lignocellulosic raw materials, high fiber yield, energy saving, low production cost, and waste The discharge of liquid is small and the pollution load is low. The biological enzymes used in the pretreatment step of bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C5/00
Inventor 徐明邢立艳
Owner 秾源科技(北京)有限公司
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