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Extraction method of resveratrol

An extraction method, the technology of resveratrol, which is applied in the field of extraction of active ingredients of traditional Chinese medicine, can solve the problems of unsatisfactory yield and purity, and achieve the effects of high yield, improved utilization rate, and improved purity

Inactive Publication Date: 2019-06-18
EAST MINGLOU BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for extracting resveratrol from Polygonum cuspidatum, but the yield and purity are not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Step 1: Wash 1kg of Polygonum cuspidatum, then dry to constant weight, and crush to 70 mesh;

[0017] Step 2: Add 11kg of ethanol with a volume fraction of 80% and ultrasonic treatment for 2.5 hours;

[0018] Step 3: Take the mixed solution after ultrasound, add 2.75kg propanol, and heat to reflux for 3 hours;

[0019] Step 4: The extract is concentrated, and the volume ratio is acetonitrile: water=3:7 for column chromatography.

[0020] Step 5: The obtained product liquid is concentrated under reduced pressure and freeze-dried to obtain a finished product.

Embodiment 2

[0022] Step 1: Wash 1kg of Polygonum cuspidatum, then dry to constant weight, and crush to 60 mesh;

[0023] Step 2: Add 10kg of ethanol with a volume fraction of 75% and ultrasonic treatment for 3 hours;

[0024] Step 3: Take the mixed solution after sonication, add 3kg propanol, and heat to reflux for 4 hours;

[0025] Step 4: The extract is concentrated, and the volume ratio is acetonitrile: water=3:7 for column chromatography.

[0026] Step 5: The obtained product liquid is concentrated under reduced pressure and freeze-dried to obtain a finished product.

Embodiment 3

[0028] Step 1: Wash 1kg of Polygonum cuspidatum, then dry to constant weight, and crush to 80 mesh;

[0029] Step 2: Add 12kg of ethanol with a volume fraction of 85%, and ultrasonic treatment for 2 hours;

[0030] Step 3: Take the mixed solution after sonication, add 2.4kg propanol, and heat to reflux for 2 hours;

[0031] Step 4: The extract is concentrated, and the volume ratio is acetonitrile: water=3:7 for column chromatography.

[0032] Step 5: The obtained product liquid is concentrated under reduced pressure and freeze-dried to obtain a finished product.

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PUM

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Abstract

The invention relates to an extraction method of active ingredients of traditional Chinese medicines, in particular to an extraction method of resveratrol. The extraction method comprises the following methods: (1), cleaning polygoni cuspidati rhizoma et radix, drying to constant weight, and smashing to 60-80 meshes; (2), adding ethyl alcohol at the volume ratio of 10-12 times the volume of the polygoni cuspidati rhizoma et radix, and performing ultrasonic treatment for 2-3 hours; (3) taking mixed liquid subjected to the ultrasonic treatment, adding propyl alcohol, and performing heating reflux for 2-4 hours; (4), concentrating an extraction solution, and performing column chromatography isolation, and (5) performing vacuum concentration on an obtained product solution and freeze-drying toobtain a finished product. According to the invention, polygoni cuspidati rhizoma et radix is subjected to the ultrasonic treatment, so that the raw material is separated effectively, the resource utilization rate is largely improved; the resveratrol is extracted through jointly performing heating reflux with propyl alcohol and ethyl alcohol, the extraction yield is high, and the column chromatography isolation is performed by acetonitrile and water, thereby improving the purity of obtained resveratrol.

Description

Technical field [0001] The invention relates to a method for extracting effective components of traditional Chinese medicine, in particular to a method for extracting resveratrol. Background technique [0002] Resveratrol is a polyphenol compound, also known as stilbene. It is a chemopreventive agent for tumors, as well as a chemopreventive agent for reducing platelet aggregation, preventing and treating atherosclerosis and cardiovascular and cerebrovascular diseases. It is derived from peanuts , Grape, knotweed, mulberry and other plants. At present, there are many methods for extracting resveratrol from Polygonum cuspidatum, but the yield and purity are not ideal. Summary of the invention [0003] The invention overcomes the shortcomings in the prior art, provides a method for extracting resveratrol, and improves the extraction yield and purity. [0004] In order to solve the above technical problems, the present invention is achieved through the following technical solutions: A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/70C07C39/21
Inventor 成小飞
Owner EAST MINGLOU BIOTECHNOLOGY CO LTD