Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation system and preparation method of rat brain injury animal model

An animal model and preparation system technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of short survival time of brain injury animal models, unstable production, and difficult removal of cushion balls, etc., to facilitate observation and manufacture Mold results, good stability, and long survival time

Pending Publication Date: 2019-06-21
肖以磊
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a preparation system of a rat brain injury animal model to solve the problems of short survival time, unstable production and difficult removal of the cushion ball in the brain injury animal model prepared in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation system and preparation method of rat brain injury animal model
  • Preparation system and preparation method of rat brain injury animal model
  • Preparation system and preparation method of rat brain injury animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This embodiment is a preparation system of a rat brain injury animal model, such as figure 1 As shown, the preparation system includes a cushion ball 1, a sleeve 2, a weight 3, and a plastic film 4. The plastic film 4 is arranged at the bottom of the sleeve 2, and the cushion ball 1 is arranged in the sleeve 2 and connected to the plastic film 4. , The weight 3 is set in the sleeve 2 and above the cushion ball 1;

[0045] Among them, the diameter of the cushion ball 1 is 2.2 mm; the inner diameter of the sleeve 2 is 1.6 cm; the weight 3 is spherical with a mass of 12 g and a diameter of 1.4 cm; the plastic film 4 is made of polyethylene; and the thickness is 0.9 dmm.

Embodiment 2

[0047] This embodiment is a preparation system of a rat brain injury animal model, such as figure 1 As shown, the preparation system includes a cushion ball 1, a sleeve 2, a weight 3, and a plastic film 4. The plastic film 4 is arranged at the bottom of the sleeve 2, and the cushion ball 1 is arranged in the sleeve 2 and connected to the plastic film 4. , The weight 3 is set in the sleeve 2 and above the cushion ball 1;

[0048] Among them, the diameter of the cushion ball 1 is 2mm; the inner diameter of the sleeve 2 is 1.7cm; the weight 3 is spherical with a mass of 14g and a diameter of 1.5cm; the plastic film 4 is made of polyethylene; and the thickness is 1dmm.

Embodiment 3

[0050] This example is a preparation method of a rat brain injury animal model. The preparation method adopts the preparation system of Example 1. The preparation method described above includes the following steps:

[0051] (a) The rats were anesthetized with 10% chloral hydrate by intraperitoneal injection, and then the rats were fixed and disinfected in the prone position, and then the scalp of the rats was incised along the median loss, at the top of the right side, bregma A bone window with a diameter of 4.5mm is opened at the rear 2mm and 2mm beside the midline;

[0052] (b) Cover the position of the bone window with plastic film, and place the cushion ball on the plastic film and face the bone window;

[0053] (c) Place the sleeve vertically above the bone window, and then put the weight into the sleeve and freely fall to hit the center of the cushion ball until the cushion ball sinks into the brain tissue 1.8mm;

[0054] (d) Take out the brain tissue from the cushion ball and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The embodiment of the invention discloses a preparation system and a preparation method of a rat brain injury animal model. The preparation system comprises a cushion ball, a sleeve, a weight and a plastic film, wherein the plastic film is arranged at the bottom in the sleeve; the cushion ball is arranged in the sleeve and is connected with the plastic film; and the weight is arranged in the sleeve and above the cushion ball. The system can realize the preparation of an animal model with brain injury, and the prepared animal model has good stability, good repeatability and long survival time;and through the arrangement of the plastic film, the system can avoided the fact that brain tissue is severely damaged and has brain edema caused by the fact that the cushion ball is directly acted onbrain tissue to penetrate through dura mater, and can further facilitate the taking out of the cushion ball.

Description

Technical field [0001] The embodiment of the present invention relates to the technical field of animal model preparation, in particular to a preparation system and preparation method of a rat brain injury animal model. Background technique [0002] Craniocerebral injury is a common trauma, which can exist alone or in combination with other injuries. Its classification is divided into scalp injury, skull injury and brain injury according to the anatomical part of the brain. The three can exist together. Scalp injuries include scalp hematoma, scalp laceration, and scalp avulsion. Skull fractures include linear fractures of the skull, skull base fractures, and depressed fractures. Brain injury includes concussion, diffuse axonal injury, brain contusion and laceration, and brain stem injury. [0003] At present, craniocerebral injury accounts for the first place in the rate of death and disability from systemic trauma. Among adolescents aged 1-34, car accidents are the first cause ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D1/00
Inventor 肖以磊祝子鹏周杰
Owner 肖以磊
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com