HPLC characteristic chromatogram of rhizome of Chinese goldthread and construction method and application thereof, and quality detection method of preparation of rhizome of Chinese goldthread

A characteristic map and construction method technology, which is applied in the quality detection of Coptidis preparations, the HPLC characteristic map of Coptis chinensis and its construction field, can solve the problem of inability to quickly and accurately judge the authenticity of Coptis chinensis, and the inability to comprehensively and effectively measure the quality of Coptis chinensis, etc. problems, to achieve the effect of stable quantity and quality transmission, good peak shape and strong operability

Inactive Publication Date: 2019-06-28
劲牌持正堂药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Coptidis medicinal materials, Coptis decoction pieces, Coptidis extract, Coptidis concentrate, Coptidis spray powder or Coptis granules exist in the market, but there is no characteristic map refle

Method used

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  • HPLC characteristic chromatogram of rhizome of Chinese goldthread and construction method and application thereof, and quality detection method of preparation of rhizome of Chinese goldthread
  • HPLC characteristic chromatogram of rhizome of Chinese goldthread and construction method and application thereof, and quality detection method of preparation of rhizome of Chinese goldthread
  • HPLC characteristic chromatogram of rhizome of Chinese goldthread and construction method and application thereof, and quality detection method of preparation of rhizome of Chinese goldthread

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preparation example Construction

[0105] In a preferred embodiment, the preparation method of test sample comprises the following steps:

[0106] (a) Add Coptidis Rhizoma Coptidis into water, boil it with strong fire, decoct it with slow fire for 15-25min, separate the filtrate and medicine dregs, add the medicine residue into water, boil it with strong fire, decoct it with slow fire for 10-20min, separate the filtrate and combine it twice filtrate;

[0107] (b) Concentrate the filtrate obtained in step (a), and obtain the test sample after drying.

[0108] In step (a), the specific operation for preparing the filtrate is not limited, and the extraction operation commonly used by those skilled in the art can be used. For example, it can be that 100g of Coptidis Rhizome is added to 900mL of water, placed in a decocting pot, and after boiling with high heat, Simmer for 20 minutes, filter through 200-mesh gauze to obtain the filtrate and medicinal residues, add the medicinal residues to 700mL of water, boil with...

Embodiment 1

[0131] Example 1 The HPLC characteristic spectrum of Coptidis rhizome

[0132] A kind of HPLC feature spectrum of Rhizoma Coptidis, HPLC feature spectrum comprises 8 characteristic peaks, with No. 8 peak berberine peak as contrast peak, the relative retention time of each characteristic peak is:

[0133] Peak No. 1: The relative retention time is 0.095;

[0134] Peak No. 2: The relative retention time is 0.359;

[0135] Peak No. 3: The relative retention time is 0.526;

[0136] Peak No. 4: The relative retention time is 0.551;

[0137] Peak No. 5: The relative retention time is 0.581;

[0138] Peak No. 6: The relative retention time is 0.653;

[0139] Peak No. 7: The relative retention time is 0.888;

[0140] Peak No. 8: The relative retention time is 1.000.

[0141] Taking No. 8 peak berberine peak as the reference peak, the relative peak area of ​​each characteristic peak is:

[0142] Peak No. 1: The relative peak area is 0.0097;

[0143] Peak No. 2: The relative pea...

Embodiment 2

[0151] Example 2 Construction of the HPLC characteristic map of Coptis Rhizoma

[0152] (1) Gather Coptis chinensis

[0153] Collect 30 batches of qualified Coptis chinensis medicinal materials from the main authentic places of Coptis chinensis such as Hubei, Sichuan, Chongqing, etc., and process each batch of medicinal materials into 23 batches of qualified Coptis chinensis pieces. Unqualified medicinal materials were directly eliminated, and the collection information of medicinal materials is shown in Table 1.

[0154] Table 1 Origin information of different batches of Coptidis Rhizoma

[0155]

[0156]

[0157] (2) preparation of need testing solution

[0158] Weigh 100g of each batch of Coptidis Rhizoma decoction pieces, put them in a decoction pot, add 900mL of pure water, boil with strong fire, then decoct with slow fire for 20min, filter the extract with 200 mesh gauze, add 700mL of pure water to the dregs, and boil with strong fire After boiling, simmer for 1...

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Abstract

The invention, which belongs to the technical field of characteristic chromatograms, provides an HPLC characteristic chromatogram of the rhizome of Chinese goldthread and a construction method and application thereof, and a quality detection method of the preparation of rhizome of Chinese goldthread. The HPLC characteristic chromatogram includes eight characteristic peaks; and the peak 8 being a berberine peak is used as a contrast peak. The relative retention time of all characteristic peaks is as follows: the relative retention time of the peak I is 0.095; the relative retention time of thepeak 2 is 0.359; the relative retention time of the peak 3 is 0.526; the relative retention time of the peak 4 is 0.551; the relative retention time of the peak 5 is 0. 581; the relative retention time of the peak 6 is 0.653; the relative retention time of the peak 7 is 0.888; and the relative retention time of the peak 8 is 1.000. The relative retention time and relative peak areas of all peaks are within a 5% range. The characteristic chromatogram can be used for controlling the quality of the preparation of rhizome of Chinese goldthread; and the operability is high.

Description

technical field [0001] The invention belongs to the technical field of characteristic maps, and in particular relates to an HPLC characteristic map of Coptidis Rhizoma, its construction method and application, and a quality detection method of Coptis Rhizoma preparations. Background technique [0002] The standard decoction follows the theory of traditional Chinese medicine, standardizes decoction according to the clinical decoction decoction method, separates the solid from the liquid, and is dried by an appropriate method. The standard decoction is prepared from no less than 15 batches of raw medicinal materials, and the relevant mean value is calculated, and the acceptable range of variation is specified. All pharmaceutical studies of traditional Chinese medicine formula granules must be compared with standard decoctions to ensure quality consistency with standard decoctions. Therefore, the quality standard research of standard decoction is particularly important for jud...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 刘源才成焕波胡辉刘鹏曾烨翟红伟龙林靳步昆
Owner 劲牌持正堂药业有限公司
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