High-flux unicell electro-spray ionization mass spectrometry device and detection method thereof
An electrospray mass spectrometry, single-cell technology, applied in the field of mass spectrometry, can solve the problems of difficult and high-throughput single-cell analysis, difficult to quickly sample and analyze, and achieve the effect of rapid single-cell analysis
Pending Publication Date: 2019-06-28
上海慧多信息科技发展有限公司
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Problems solved by technology
The disadvantage of this invention is that it can only be used for rapid sampling and analysis of liquids. It is difficult to achieve accurate and rapid sampling and analysis of extremely small volumes of single cells in liquids, and it is even more difficult to achieve high-throughput single-cell analysis.
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Embodiment 1
[0031] Such as figure 1 As shown, the high-throughput single-cell electrospray mass spectrometry device of the present invention includes a support frame 3, and a first three-way pipe 4 and a second three-way pipe 9 connected in series are horizontally fastened in the support frame to form a sample in the horizontal direction. channel, the first long straight capillary needle 1, the second long straight capillary needle 8, and the third long straight capillary needle 12 are arranged on the sample channel, and the left end of the first long straight capillary needle 1 protrudes from the sample channel Outside, the right end is arranged in described sample channel, near the right end of the first three-way pipe 4, and the clamping device 6 that connects two three-way pipes is set between the left end of the second three-way pipe 9, the first three-way pipe 4 is connected to the syringe pump 5 through a pipeline, and a mass spectrometer 14 is arranged on the right side of the thi...
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Abstract
The invention relates to a high-flux unicell electro-spray ionization mass spectrometry device and a detection method thereof. The device comprises a bracing frame, first and second three-way pipes inserial connection are horizontally fastened into the bracing frame, a sample channel is formed in the horizontal direction, first, second and third long straight capillary tube needles are arranged in the sample channel, the left end of the first long straight capillary tube needle extends out of the sample channel, the right end of the first long straight capillary tube needle is arranged in thesample channel and close to the right end of the first three-way pipe, a clamping device for connecting the two three-way pipes is arranged between the left end of the first long straight capillary tube needle and the left end of the third long straight capillary tube needle, the first three-way pipe is connected with an injection pump via a pipeline, and a mass spectrometer is arranged on rightof the third long straight capillary tube needle. Via the device, the cell order is analyzed in the mass spectrometer, and the unicell can be analyzed rapidly by means of the rapid mass spectrum analysis capability and capability of detecting thousands of substances in single time.
Description
technical field [0001] The invention belongs to the field of mass spectrometry analysis, in particular to a high-throughput single-cell electrospray mass spectrometry device and a detection method thereof. Background technique [0002] As a highly sensitive and fast analytical instrument, mass spectrometry has been widely used in medicine, biology, food, chemistry and other fields. Single-cell analysis is a direction of great concern in modern biology and medicine. Cells are heterogeneous, and cells in the same part of the same living body are different, which is also a reason for cancer. There have been many reports on mass spectrometry analysis of substances in a single cell and their metabolic processes, but the analysis speed is slow, and it is difficult to analyze and count a large number of cells, which makes cell research and medical analysis difficult. It's difficult. The latest ICP-MS method for single-cell analysis can reach 1000 cells per minute, but it is limit...
Claims
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Login to View More IPC IPC(8): H01J49/16G01N27/62
Inventor 俞俊杰蒋红
Owner 上海慧多信息科技发展有限公司