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VHH (variable domain of heavy chain of heavy-chain antibody) chain of IL23 single domain antibody, IL23 single domain antibody, nucleotide sequence and kit

A nucleotide sequence and single-domain antibody technology, applied in the field of genetic engineering, can solve the problems of low expression and low antibody activity

Pending Publication Date: 2019-07-05
SHENZHEN PREGENE BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the IL-23 antibody in the prior art has low activity and low expression level

Method used

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  • VHH (variable domain of heavy chain of heavy-chain antibody) chain of IL23 single domain antibody, IL23 single domain antibody, nucleotide sequence and kit
  • VHH (variable domain of heavy chain of heavy-chain antibody) chain of IL23 single domain antibody, IL23 single domain antibody, nucleotide sequence and kit
  • VHH (variable domain of heavy chain of heavy-chain antibody) chain of IL23 single domain antibody, IL23 single domain antibody, nucleotide sequence and kit

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Embodiment Construction

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0022] The present invention provides an IL23 single domain antibody, nucleotide sequence and kit. The following content will introduce the polypeptide and its screening process in detail.

[0023] First, the polypeptide of the present invention has three framework regions and two variable regions:

[0024] FR1: Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly GlySer Leu Arg Leu Ser Cys Ala Ala Ser Gly Trp Asn Leu Gly;

[0025] CD...

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Abstract

The invention discloses a VHH (variable domain of heavy chain of heavy-chain antibody) chain of IL23 single domain antibody, IL23 single domain antibody, a nucleotide sequence and a kit, wherein an amino acid sequence of the VHH chain of the IL23 single domain antibody is shown as SEQ ID NO: 1. The single domain antibody specifically binding with IL23 protein is attained herein; the single domainantibody has high activity in specific binding with IL23 protein, has high expression and low cost and is easy to modify.

Description

Technical field [0001] The present invention relates to the technical field of genetic engineering, in particular to a VHH chain of an IL23 single domain antibody, an IL23 single domain antibody, a nucleotide sequence and a kit. Background technique [0002] IL-23 is a heterodimeric cytokine and a member of the IL-12 family. IL-23 is mainly secreted by activated dendritic cells and macrophages, which can promote the proliferation of T cells and the production of IFN-γ, and has strong anti-tumor activity and immune protection against infection. Studies have shown that high expression of IL-23 can cause a variety of autoimmune diseases, such as psoriasis, rheumatoid arthritis, systemic lupus erythematosus, inflammatory bowel disease, multiple sclerosis, and central nervous system autoimmune inflammation. [0003] IL23 antagonists (including, for example, anti-IL23 antibodies or antigen-binding fragments thereof that target, for example, the p19 subunit of IL23) can effectively reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/24C12N15/13
CPCC07K16/244C07K2317/569C07K2317/92
Inventor 李胜华李莹莹包朝乐萌许莎莎余祥刘文涛
Owner SHENZHEN PREGENE BIOPHARMA CO LTD