A sample pretreatment method for endogenous brassinosterol in plant samples and the pretreatment solid-phase material used therefor

Abstract

The invention discloses a sample pretreatment method of endogenous brassinosterol in plant samples and a pretreatment solid phase material used therefor. In this method, the endogenous brassinosterol is firstly extracted with a solvent, and the brassinosterol in the obtained extraction solution is derivatized and simultaneously extracted with a solid-phase material adsorbed with a boron affinity reagent, and then salt is introduced simultaneously during the desorption to induce phase transfer extraction. , further remove excess boron affinity reagent and hydrophilic impurities, and then realize the selective enrichment of endogenous brassinosterol in plant samples. The method is simple, fast, highly sensitive, and high-throughput, especially in the removal of endogenous impurities in plant extracts, showing high selectivity, and can realize the pretreatment of low-level endogenous brassinosteroids, combined with chemical derivatization, and then Improved analytical sensitivity for the detection of endogenous brassinosteroids in plant samples.

Description

technical field [0001] The invention relates to a sample pretreatment method for selectively enriching endogenous brassinosteroids in plant samples and a pretreatment solid-phase material, belonging to the field of analytical chemistry. Background technique [0002] Brassinosteroids (BRs) are a class of polyhydroxylated sterol compounds, which are found in extremely low levels in plants, but they regulate a series of physiological and metabolic processes in plants. In recent years, studies on the physiological functions of brassinosterols, such as the biosynthesis, degradation and metabolic pathways of BRs, have received extensive attention. However, due to the complex matrix and extremely low brassinosterol content in plant extracts, the development of these studies is limited. Therefore, establishing an efficient sample pretreatment method combined with a sensitive detection method is the key to the detection of endogenous BRs. [0003] At present, the method based on li...

AI Technical Summary

Problems solved by technology

Although the selectivity has been greatly improved, the synthesis of materials is complicated and cumbersome, and the desorption between BR and materials is difficult, often requiring the introduction of oxidants such as hydrogen peroxide

For example, when extracting BR in CN2013101314228, it is necessary to use phenylboronic acid magnetic nanomaterials synthesized in advance, and it is necessary to use a desorption liquid to desorb BR, and the process is relatively cumbersome

[0005] Second, due to the poor ionization efficiency of BR, the fragmentation behavior on the induced collision dissociation (CID) in the secondary mass spectrometry is not good, resulting in poor signal response and low sensitivity on MS

However, there are still problems: in order to ensure that the derivatization reaction is fully carried out, a large excess of derivatization reagents relative to the analyte is generally introduced, which will interfere with the detection of the analyte

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