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A kind of micropatterned nanofiber material composited with drug-loaded MOF and its preparation method and application

A technology of micropatterning and nanofibers, which is applied in the fields of pharmaceutical formulations, fiber chemical characteristics, cellulose/protein conjugated artificial filaments, etc., can solve problems such as increasing red blood cell production, and achieve wound repair, safe healing, and growth promotion active effect

Active Publication Date: 2021-11-19
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of DMOG has side effects, such as promoting massive angiogenesis leading to tumor formation and increasing red blood cell production, etc.

Method used

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  • A kind of micropatterned nanofiber material composited with drug-loaded MOF and its preparation method and application
  • A kind of micropatterned nanofiber material composited with drug-loaded MOF and its preparation method and application
  • A kind of micropatterned nanofiber material composited with drug-loaded MOF and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1 Preparation of Micropatterned Nanofiber Membrane

[0083] (1) Preparation and morphological characterization of ZIF-67 (zeolitic imidazole framework) and ZIF-67 loaded with DMOG (dimethyloxalylglycine)

[0084] 1. Preparation of ZIF-67 nanoparticles

[0085] Dissolve 0.291g of cobalt nitrate hexahydrate and 0.66g of 2-methylimidazole in 15mL of methanol respectively, mix and stir at room temperature for 24h, centrifuge at 10,000rpm for 5min to obtain the product, wash repeatedly with methanol, and vacuum-dry at 80°C for 24h to obtain ZIF-67 nanoparticles, its SEM, TEM, EDS, XRD, nitrogen adsorption and desorption curves are as follows figure 1 shown.

[0086] 2. Preparation of drug-loaded ZIF-67 nanoparticles

[0087] At 37°C, 25 mg of ZIF-67 was soaked in DMOG phosphate buffered saline solution (PBS, pH=7.4) with a concentration of 15 mg / mL for 3 days, centrifuged and washed, and then vacuum-dried at 37°C for 24 hours to obtain loaded DMOG The ZIF-67 (DMO...

Embodiment 5

[0124] Example 5 Full-thickness wound repair experiment in vivo

[0125] 30 18g male BALB / c experimental mice were induced to suffer from diabetes by STZ, blood glucose was measured one week after induction, the blood glucose value increased and the value was stable at 250mg / dl, indicating that the mice were successfully induced into diabetic mice for subsequent experiments .

[0126] Establish a full-thickness skin wound (circular, 8mm in diameter) in the back area of ​​diabetic mice, and implant the micropatterned nanofiber materials prepared in Example 1 in the mouse wound area and group them, respectively: blank Control group (Control), PL / G group, Z-PL / G group, DZ-PL / G group. On days 0, 3, 7, 9 and 11, digital cameras were used to record the wound healing changes in the trauma area at fixed distances and angles, such as Figure 8 a. according to Figure 8 A, the effect diagram of the simulated effect of wound repair on the back of diabetic mice Figure 8 B, Statistic...

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Abstract

The invention belongs to the field of biological materials, and relates to a micropatterned nanofiber material compounded with drug-loaded MOF, a preparation method and application thereof. The material is based on hydrophilic composite electrospun nanofibers that are staggered with each other as the skeleton, and the micro-patterned structure is arranged in an orderly manner, and an open three-dimensional porous structure is distributed; the hydrophilic composite electrospun nanofibers are supporting biological A blend of compatible materials, hydrophilic biocompatible materials, and metal-organic framework nanoparticles loaded with repairing active molecules. The preparation method is as follows: using an organic solvent uniformly dispersed with metal-organic framework nanoparticles loaded with repairing active molecules, supporting biologically active materials and hydrophilic biologically active materials as spinning liquid, and electrostatically spinning to prepare. The material is based on the activity of the slow-release drug carrier and the activity of biological tissue, and synergistically promotes the activity of cells in the body environment and the micropatterned nanofiber material, which is beneficial to tissue repair and wound healing, and is used to prepare wound repair materials.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a micropatterned nanofiber material compounded with drug-loaded MOF, a preparation method and application thereof. Background technique [0002] Foot ulcers are one of the main complications of diabetes, and the wounds are protracted. In severe cases, toe / limb amputation is required, and the postoperative five-year mortality rate is as high as 50% to 68%. Normal skin wounds can generally self-repair in a timely and orderly manner, while diabetic wounds have insufficient angiogenesis and slow wound healing, which is mainly due to the decreased secretion of angiogenesis-promoting growth factors and the decreased expression of their corresponding receptors in high-glucose environments , the matrix deposition is reduced, and the microenvironment required for wound healing cannot be formed. [0003] Skin tissue engineering, which has emerged in recent years, uses synth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/54A61L27/56A61L27/00A61L27/22A61L27/18D01F8/14D01F8/02D01F1/10
CPCA61L27/00A61L27/18A61L27/222A61L27/227A61L27/54A61L27/56A61L27/60A61L2300/102A61L2300/214A61L2300/41A61L2300/412A61L2300/414A61L2300/45A61L2300/602A61L2400/12D01F1/10D01F8/02D01F8/14C08L89/00C08L67/04
Inventor 徐合柯勤飞李锦绣吕方张天帅徐雅凤
Owner SHANGHAI NORMAL UNIVERSITY
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