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Marker for fever of unknown origin, and detection method and application of marker

A marker and cause technology, applied in the field of biomedicine, can solve problems such as abnormal activation

Pending Publication Date: 2019-07-16
JIANGSU MICROMEDMARK BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the human body does not have any symptoms after being infected with HCMV, but when the human immune system is suppressed / disordered, HCMV can be abnormally activated and cause a variety of diseases

Method used

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  • Marker for fever of unknown origin, and detection method and application of marker
  • Marker for fever of unknown origin, and detection method and application of marker
  • Marker for fever of unknown origin, and detection method and application of marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Screening of response miRNA in serum / plasma of patients with fever of unknown origin

[0143] The present invention collected (waste serum collected from the laboratory department of Nanjing General Hospital) 10 cases of normal people, 4 cases of patients with vasculitis, and 10 cases of patients with fever of unknown origin. The expression level of HCMV miRNA in serum / plasma of patients with febrile cause.

[0144] The result is as figure 1 As shown, the expression levels of various HCMV miRNAs in serum / plasma of patients with vasculitis were higher than those of normal controls, such as: miR-UL36-3p, miR-UL36-5p, miR-US4-3p, miR-US4-5p, miR-US22 -3p, miR-US-29-3p, miR-US33-5p, miR-UL59; the expression levels of various HCMV-responsive miRNAs in serum / plasma of patients with unexplained fever were higher than those of normal controls, such as: miR-UL36-3p, miR - UL36-5p, miR-US4-3p, miR-US4-5p, miR-US22-3p, miR-US-29-3p, miR-US33-5p, miR-UL59. The results show that ...

Embodiment 2

[0146] Validation of miRNA markers in patients with fever of unknown origin

[0147] In order to verify the effect of HCMV miRNA, the expression of HCMV miRNA in normal people and patients with unexplained fever (the patient showed fever and the cause of the disease is unclear) in Example 1 was further analyzed. The basic information of the patients is shown in Table 1.

[0148] Table 1 Basic information of patients used in the experiment

[0149]

[0150] Then, the levels of all HCMV miRNAs (26 species in total, miRbase 19.0) in serum / plasma of the above patients were detected by using Real-time PCR technology.

[0151] The result is as figure 2 As shown, a variety of HCMV miRNAs in patient serum / plasma, such as hcmv-miR-UL148D, hcmv-miR-UL22-5p, hcmv-miR-UL36-3p, hcmv-miR-US33-5p, hcmv-miR-US5, The expression levels of hcmv-miR-UL122-5p and hcmv-miR-US25-5p are higher than those of normal controls. This result once again confirms that HCMV miRNA may play an important r...

Embodiment 3

[0153] Validation / identification of responsive miRNAs

[0154] In order to further explore the physiological function of HCMV miRNA and its relationship with the occurrence and development of some unexplained fever and unexplained immune diseases (vasculitis, rheumatic fever, primary biliary cirrhosis, etc.). Using bioinformatics methods (http: / / www.targetscan.org / ) to predict the potential human target genes of HCMV miRNA

[0155] The results of bioinformatics prediction are shown in Table 2 and image 3 , found that HCMV-encoded miRNA can regulate the expression of a variety of human genes.

[0156] Table 2 The number and potential target genes of HCMV miRNAs predicted by bioinformatics to regulate human target genes

[0157]

[0158]

[0159] From Table 2 and image 3 It can be seen that through the analysis of the above target genes, a variety of HCMV miRNA target genes are related to apoptosis, proliferation and immune response (such as BCL2L11, CDC42, PDCD4, CCN...

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Abstract

The invention provides a marker for fever of unknown origin (FUO), and a detection method and application of the marker, and specifically, provides a FUO specificity marker and the purpose thereof fordetecting a reagent. The marker is used for preparing a diagnosis reagent or a diagnosis reagent kit. The diagnosis reagent or reagent kit is used for (i) judging whether a detected object suffers from fever of unknown origin or not, and / or (ii) judging the risk (infectibility) that the detected objects suffers from the fever of unknown origin. The invention also provides a reagent and reagent kit for detecting the fever of unknown origin, and a method for diagnosing the fever of unknown origin.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more specifically to markers, detection methods and applications of fever of unknown origin. Background technique [0002] Fever of unknown origin (FUO) refers to fever that lasts for more than 3 weeks, with body temperature above 38.3°C for many times, and cannot be clearly diagnosed after detailed medical history, physical examination, and routine laboratory tests. It is a relatively common clinical symptom or disease, which can occur in all age groups, and is more common in the afternoon or evening. The key to the diagnosis of fever of unknown origin is to find out the cause. From a diagnostic point of view, fever can be divided into four categories: infectious diseases, malignant diseases, connective tissue diseases, inflammatory vascular diseases, and other diseases that can cause fever. Among them, infectious diseases are the most common, accounting for about 60-70% of all fevers of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/70C12N15/11C40B40/06A61K45/00A61P31/22A61P29/00
CPCC12Q1/6883C12Q1/701A61K45/00C12Q2600/166C12Q2600/172
Inventor 张辰宇王成张春妮
Owner JIANGSU MICROMEDMARK BIOTECH