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Functionalized oriented fibers for tissue-engineered vascular scaffold construction and preparation method thereof

A functionalization and orientation technology, applied in fiber treatment, fiber type, pharmaceutical formulation, etc., can solve the problems of lack of activity of polyamino functional groups and complicated preparation process, and achieve the effect of enhancing application efficacy, simple process, and enhancing coating effect

Active Publication Date: 2021-07-02
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a defect in the prior art that it is necessary to further introduce active groups on the basis of the PDA coating for grafting, the preparation process is complicated, and the introduced polyamino functional groups lack activity. Lys improves the coating effect of PDA, and the oriented fiber prepared by synthetic polymer is modified to prepare the oriented fiber modified by PDA / Lys

Method used

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  • Functionalized oriented fibers for tissue-engineered vascular scaffold construction and preparation method thereof
  • Functionalized oriented fibers for tissue-engineered vascular scaffold construction and preparation method thereof
  • Functionalized oriented fibers for tissue-engineered vascular scaffold construction and preparation method thereof

Examples

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Effect test

Embodiment 1

[0039] At room temperature, dissolve 0.57 g of PLCL (the copolymerization ratio of lactic acid and caprolactone is 50:50, and the viscosity is 2.9 dl / g) in 6 mL of hexafluoroisopropanol, and stir for 12 hours to obtain a mass fraction of 10% Spinning solution: Electrospinning is carried out at room temperature and humidity of 40-60%, the applied voltage is 5kV, the receiving distance is 20cm, the drum receiving speed is 1000rpm, and the injection rate is 0.5mL / h.

[0040] The scanning electron micrographs of the prepared pure PLCL oriented fibers, such as figure 1 As shown, it shows that it has a highly oriented structure, and the fiber diameter is 1.67±0.17 μm.

[0041] Cell adhesion after 3 hours of planting ECs on the scaffold, such as Figure 7As shown, the expression of the cell function protein VE-Cadherin after the scaffold was planted with ECs for 3 days, as shown in Figure 10 As shown, although PLCL-oriented fibers can maintain the functional expression of ECs, th...

Embodiment 2

[0044] At room temperature, dissolve 0.57 g of PLCL (the copolymerization ratio of lactic acid and caprolactone is 50:50, and the viscosity is 2.9 dl / g) in 6 mL of hexafluoroisopropanol, and stir for 12 hours to obtain a mass fraction of 10% Spinning solution: Electrospinning is carried out at room temperature and humidity of 40-60%, the applied voltage is 5kV, the receiving distance is 20cm, the drum receiving speed is 1000rpm, and the injection rate is 0.5mL / h. Dissolve 20 mg of DA and 2 mg of Lys in 10 mL of Tris buffer solution with a concentration of 10 mM and a pH of 8.5, stir and dissolve to prepare a DA / Lys solution. A 1 cm×1 cm oriented fiber-based scaffold was immersed in the DA / Lys solution, and vibrated at room temperature for 24 h at a vibration rate of 100 times / min to obtain PDA / Lys modified oriented fibers. SEM images of oriented fibers modified by PDA / Lys, such as image 3 As shown, the granular aggregates on the fiber surface began to increase, and the furth...

Embodiment 3

[0046] At room temperature, dissolve 0.57 g of PLCL (the copolymerization ratio of lactic acid and caprolactone is 50:50, and the viscosity is 2.9 dl / g) in 6 mL of hexafluoroisopropanol, and stir for 12 hours to obtain a mass fraction of 10% Spinning solution: Electrospinning is carried out at room temperature and humidity of 40-60%, the applied voltage is 5kV, the receiving distance is 20cm, the drum receiving speed is 1000rpm, and the injection rate is 0.5mL / h. Dissolve 20 mg of DA and 10 mg of Lys in 10 mL of Tris buffer solution with a concentration of 10 mM and a pH of 8.5, stir and dissolve to prepare a DA / Lys solution. A 1 cm×1 cm oriented fiber-based scaffold was immersed in the DA / Lys solution, and vibrated at room temperature for 24 h at a vibration rate of 100 times / min to obtain PDA / Lys modified oriented fibers. SEM images of oriented fibers modified by PDA / Lys, such as Figure 4 As shown, it shows that the granular aggregates on the fiber surface begin to decreas...

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Abstract

The invention relates to a functionalized oriented fiber for tissue engineering vascular stent construction and a preparation method thereof. A DA / Lys solution is obtained by adding dopamine salt DA and lysine Lys into a buffer solution; immersing the oriented fiber in DA / Lys solution reaction, that is. The invention not only effectively enhances the coating effect of PDA by introducing the active ingredient Lys, but also significantly improves the function of inducing vascular endothelial regeneration of oriented fibers after coating modification. The PDA / Lys coating method provided by the present invention has simple process and high efficiency, can effectively solve the defects of poor biocompatibility and insufficient function of inducing endothelial regeneration of polymer-based tissue engineered vascular stents, and promote the research and development of functional tissue engineered blood vessels Transformation with clinical application.

Description

technical field [0001] The invention belongs to the field of functionalized oriented fiber and its preparation, in particular to a functionalized oriented fiber used for tissue engineering vascular support construction and its preparation method. Background technique [0002] The oriented fibers prepared by electrospinning are considered to be an important class of biomimetic scaffold construction materials that can regulate the behavior of vascular cells and promote the repair and functional regeneration of damaged blood vessels because of their structural characteristics of biomimetic natural vascular tissue extracellular matrix. Synthetic polymers are widely used in the preparation of oriented fiber scaffolds in the field of vascular tissue engineering due to their easy processing, controllable properties, and wide range of material sources, but they have poor biocompatibility and do not have the ability to regulate vascular cell adhesion, proliferation, and differentiatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F6/84D06M15/37D06M13/342A61L27/18A61L27/50D06M101/32
CPCA61L27/18A61L27/50A61L27/507A61L2400/18D01F6/84D06M13/342D06M15/37D06M2101/32C08L67/04
Inventor 张彦中易兵成沈炎冰唐寒王先流
Owner DONGHUA UNIV
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