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Quick identifying method of germplasm materials resistant or tolerant to citrus Huanglongbing

A technology of citrus Huanglongbing and identification method, which is applied in the field of rapid identification of germplasm materials, can solve the problems of a small number of inoculated pathogens, a long time, a small size, etc., and achieves the elimination of inoculation efficiency and success rate, accurate results, and identification efficiency. high effect

Inactive Publication Date: 2019-07-23
广西特色作物研究院
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Problems solved by technology

The main disadvantage of the above method is that each material to be identified can only be inoculated with pathogens by grafting. Due to the small size of the grafted materials, the number of pathogens contained is small, so that the number of inoculated pathogens is small. In addition, because the identification period is usually more than 14 months, Long time and low efficiency

Method used

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  • Quick identifying method of germplasm materials resistant or tolerant to citrus Huanglongbing
  • Quick identifying method of germplasm materials resistant or tolerant to citrus Huanglongbing
  • Quick identifying method of germplasm materials resistant or tolerant to citrus Huanglongbing

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Embodiment

[0073] Step 1: Collection of germplasm to be identified as resistant or tolerant to citrus HLB

[0074] From 2014 to 2018, the germplasm materials with resistance or tolerance to citrus Huanglongbing were investigated, excavated and collected in Guangxi. A total of 62 germplasm materials to be identified were collected, including 4 pomelo species, 7 orange species, 43 tangerine species and 8 mandarin species. The above-mentioned 62 parts of germplasm materials were grafted on Hovenia trifoliata anvils respectively, and planted in the greenhouse of Guangxi Specialty Crops Research Institute Citrus Resource Nursery (such as figure 1 Shown), the growth condition is better, can meet collection scion and grafting use, and carry out PCR detection.

[0075] Step 2: Select citrus trees that have grafting affinity for the germplasm materials to be identified in step 1 and are infected with H.

[0076] Citrus trees infected with Huanglongbing were selected in citrus orchards and confi...

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Abstract

The invention discloses a quick identifying method of germplasm materials resistant or tolerant to citrus Huanglongbing and belongs to the technical field of agriculture. The method includes followingsteps: step 1, collecting a to-be-identified germplasma material; step 2, selecting a citrus tree having grafting affinity to the to-be-identified germplasma material in the step 1 and infected withHuanglongbing pathogen as a top grafting tree; step 3, performing top grafting; step 4, performing field management; step 5, observing field symptoms; step 6, performing real-time fluorescent quantitative PCR (polymerase chain reaction) detection; step 7, performing result evaluation. It is the first time to directly top graft the to-be-identified germplasma material onto the tree with Huanglongbing for identifying resistance or tolerance, and there are no other related similar reports at home and abroad.

Description

technical field [0001] The invention relates to a rapid identification method for germplasm materials with resistance or tolerance to citrus huanglongbing, belonging to the field of agricultural technology. Background technique [0002] Huanglongbing (Huanglongbing) is a worldwide destructive bacterial disease of citrus. Its pathogen is a phloem-restricted and difficult-to-cultivate bacterium. So far, three species have been found: Asian species (Candidatus Liberibacterasiaticus), African species (Ca.L. africanus) and American species (Ca.L.americanus), among which the Asian species is the most widespread, and the Asian species is also popular in my country. Huanglongbing causes billions of economic losses to my country's citrus industry every year. [0003] In the early stage of Huanglongbing infection, the newly drawn branches and leaves of the plants stop turning green when they are close to maturity, and obvious "yellow shoots" are formed on the top of the crown. The aff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30C12Q1/689C12Q1/6851C12Q1/06
CPCA01G2/30C12Q1/6851C12Q1/689C12Q2531/113C12Q2545/114C12Q2563/107
Inventor 邓崇岭唐艳娄兵海武晓晓刘萍陈传武牛英张戈壁
Owner 广西特色作物研究院
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