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A method for measuring aspartame content based on surface-enhanced Raman scattering

A surface-enhanced Raman and aspartame technology, applied in the field of edible sweetener detection, can solve the problems of complex production process and unsuitable sweetener detection, and achieve the effect of simple operation, low cost and sensitive detection

Active Publication Date: 2020-09-04
JIANGNAN UNIV
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Problems solved by technology

However, the currently reported detection methods for PDMS-modified substrates are to attach nano-metal ions to the surface of cured PDMS by evaporation method, or to mix nano-scale metals obtained by reduction method into PMDS for curing. The production process is relatively complicated, and The inventors found that the substrates prepared by these methods only have a certain detection effect on certain substances, and are not suitable for the detection of sweeteners

Method used

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  • A method for measuring aspartame content based on surface-enhanced Raman scattering
  • A method for measuring aspartame content based on surface-enhanced Raman scattering
  • A method for measuring aspartame content based on surface-enhanced Raman scattering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Making PDMS plasma cavity:

[0049]a. Weigh an appropriate amount of reagents A and B of Sylgard-184 and mix them in a mass ratio of 10:1. The above mixture was vigorously stirred in a beaker for about 20 minutes, then ultrasonically oscillated for 10 minutes, and then placed in a vacuum to remove air bubbles in the mixture. Thereafter, the prepared PDMS was cast and fixed in a petri dish (diameter 35 mm, height 17 mm).

[0050] b. Prepare a silver nitrate solution with a concentration of 50 μg / mL, and slowly add 100 μg of the solution to the upper surface of the prepared liquid PDMS dropwise with a pipette gun. Since the surface tension of water is higher than that of liquid PDMS (γ water About 72.8mN / m, γ liqPDMS It is about 22~25mN / m), and the moment the silver nitrate solution is dropped into the liquid PDMS, a silver nitrate solution sphere is formed (such as figure 2 shown).

[0051] c. Place the petri dish in an environment of 25°C for 72 hours to cure ...

Embodiment 2

[0056] Embodiment 2: determination of aspartame content in the test sample

[0057] With reference to the method in Example 1, the test sample of 0.035mg / L is detected, and its Raman spectrum is as follows Figure 7 shown. The sample to be tested is at the characteristic peak of 1590.3cm -1 The divided peak intensity value was 552.65, and the content of aspartame in the sample to be tested was calculated to be 0.03852595871 mg / L through linear model calculation, with an accuracy rate of 89.93%.

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Abstract

The present invention discloses a method for measuring a content of aspartame based on surface enhanced Raman scattering, and belongs to the technical field of edible sweetener detection. In the method of the present invention, the surface enhanced Raman scattering technology is combined with a PDMS plasma chamber to measure a Raman spectrum of a concentration of aspartame, and a peak intensity value of the characteristic peak at 1588+ / -5 cm<-1> and the content of aspartame to construct a linear model used for measurement of the concentration of aspartame. Quantitative detection is more feasible, and can detect aspartame in the low concentration range of 0.002mg / L to 0.04mg / L, and a measurement coefficient reaches 0.96 with more accuracy.

Description

technical field [0001] The invention relates to a method for measuring aspartame content based on surface-enhanced Raman scattering, and belongs to the technical field of edible sweetener detection. Background technique [0002] Aspartame (N-L-α-Aspartyl-L-phenylalanine methyl ester) is a low-calorie artificial sweetener that is 150-200 times sweeter than sugar. It is widely used in many foods and beverages, especially soft drinks. Aspartame is currently permitted as an additive in food and beverages in more than 100 countries. The acceptable limit of aspartame is estimated to be 50mg / kg of body weight; the maximum allowable concentration of aspartame in soft drinks is 600mg / L. However, the metabolites of aspartame, phenylalanine and methanol, have the potential to poison consumers if they are present in high levels in foods and beverages. [0003] At present, there are many methods for detecting and quantifying aspartame in food, such as high-pressure liquid chromatograp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陈国庆陈律铭吴亚敏李磊朱纯马超群朱焯炜辜姣高辉朱拓
Owner JIANGNAN UNIV
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