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Microfluidic chip for constructing bionic lung cancer brain metastasis model and model construction method

A microfluidic chip and brain transfer technology, applied in biochemical equipment and methods, biochemical instruments, biomass post-processing, etc., can solve problems such as the inability to effectively build the blood-brain barrier and the inability to realize dynamic real-time bionic lung cancer brain metastasis.

Active Publication Date: 2019-07-26
THE SECOND HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problem that the microfluidic chip in the prior art cannot effectively construct the blood-brain barrier, nor can it realize the dynamic and real-time bionic lung cancer brain metastasis process, the present invention provides a microfluidic chip and a model construction method for building a bionic lung cancer brain metastasis model

Method used

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  • Microfluidic chip for constructing bionic lung cancer brain metastasis model and model construction method
  • Microfluidic chip for constructing bionic lung cancer brain metastasis model and model construction method
  • Microfluidic chip for constructing bionic lung cancer brain metastasis model and model construction method

Examples

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Embodiment 1

[0070] Example 1 A microfluidic chip for the construction of a bionic lung cancer brain metastasis model

[0071] A microfluidic chip constructed by a bionic lung cancer brain metastasis model, which includes a bionic lung part 01 and a bionic brain part 04, characterized in that the bionic lung part 01 is arranged upstream of the chip, which includes a three-layer structure, respectively The upper substrate layer 1, the middle porous film layer 2 and the lower substrate layer 3; the upper substrate layer 1 is provided with a gas channel 11 for air circulation, an inlet 53 and an outlet 54 for the gas supply liquid to enter and exit, and the upper half of the vacuum channel Part 12; the lower substrate is provided with a liquid passage 31 for liquid circulation, an inlet 53 and an outlet 54 for liquid to enter and exit, and a lower half 32 of the vacuum passage; the gas passage 11 and the liquid passage 31 are arranged between the upper substrate layer 1 and the lower substrate...

Embodiment 2

[0088] Example 2 A microfluidic chip for the construction of a bionic lung cancer brain metastasis model

[0089]A microfluidic chip constructed by a bionic lung cancer brain metastasis model, which includes a bionic lung part 01 and a bionic brain part 04, characterized in that the bionic lung part 01 is arranged upstream of the chip, which includes a three-layer structure, respectively The upper substrate layer 1, the middle porous film layer 2 and the lower substrate layer 3; the upper substrate layer 1 is provided with a gas channel 11 for air circulation, an inlet 53 and an outlet 54 for the gas supply liquid to enter and exit, and the upper half of the vacuum channel Part 12; the lower substrate is provided with a liquid passage 31 for liquid circulation, an inlet 53 and an outlet 54 for liquid to enter and exit, and a lower half 32 of the vacuum passage; the gas passage 11 and the liquid passage 31 are arranged between the upper substrate layer 1 and the lower substrate....

Embodiment 3

[0102] Example 3 A method for constructing a bionic lung cancer brain metastasis model

[0103] Upstream bionic lung construction:

[0104] The chip is pre-buried so that cells can better attach to the porous membrane surface of the chip. Dilute BME (Cultrex basement membrane extract, R&D Systems, Mckinley PlaceN, USA) at a ratio of 1:10, mix well, inject into the sample inlet of the microfluidic chip with a micro-sampler, and wait for the gel to solidify overnight in the incubator.

[0105] For the culture of macrophages, monocyte THP-1 was treated with 10 3 cells / cm 2 The cell density is resuspended in phorbol ester PMA (100pg / m1), injected through the inlet of the lower channel with a microsampler, and the excess will be discharged from the outlet. Tilt the chip 30 degrees to one side to allow monocytes to settle to one side of the central culture channel, place at 37°C, 5% CO 2 Incubator culture. After 48h, the monocytes were stimulated into macrophages. The chip was...

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Abstract

The invention provides a microfluidic chip for constructing a bionic lung cancer brain metastasis model to solve the problem of the current difficulty in constructing a blood brain barrier for the lung cancer brain metastasis and incapability to achieve intuitive observation and the like. The microfluidic chip comprises a bionic lung portion and a bionic brain portion and is characterized in thatthe bionic lung portion is disposed on the upstream of the chip and comprises a three-layer structure including an upper substrate layer, an intermediate porous film layer and a lower substrate layer.The three-layer structure is provided with a gas channel, a liquid channel and a vacuum channel. The bionic brain portion is disposed on the downstream of the chip, and comprises a two-layer structure including an upper substrate layer and a lower substrate layer. The upper substrate layer is a cover layer. The lower substrate layer comprises a brain parenchyma chamber, and a blood brain barrierring is arranged outside the brain parenchyma chamber. A blood vessel cavity is arranged outside the blood brain barrier ring. The blood vessel cavity is connected with the liquid channel on the lowersubstrate layer of the bionic lung portion through a connecting channel. The blood brain barrier ring is provided with a micro gap structure communicated with the brain parenchyma chamber and the vascular chamber. The vascular chamber and the brain parenchyma chamber are provided with an inlet and an outlet for the liquid to flow in and out.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chip and model construction for cancer metastasis, in particular to a microfluidic chip and a model construction method applied in the field of lung cancer brain metastasis, and specifically relates to a microfluidic chip and a model constructed for a bionic lung cancer brain metastasis model. Model building method. Background technique [0002] Brain metastasis of lung cancer is a series of complex pathophysiological cascade processes, which can be roughly divided into "three steps" - the occurrence and development of tumor in the lung in situ, tumor cells invade the pulmonary blood vessels and enter the circulatory system to become circulating tumor cells, and the circulating tumor cells remain Brain metastases develop from brain microvessels and break through the blood-brain barrier into the brain parenchyma. Due to the particularity of the anatomy and function of the central nervous syst...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/00
CPCC12M21/08C12M23/16
Inventor 王琪刘雯雯宋晶杜小慧周洋李扬李恩成徐明鑫郝俊霞
Owner THE SECOND HOSPITAL OF DALIAN MEDICAL UNIV
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