Fast fermentation compound probiotic regulator for improving water quality of aquaculture farm

A technology of composite probiotics and regulators, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problem of loss of efficacy of microecological preparations, costly settings, and absence of regulators, etc. problem, to achieve the effect of promoting aquatic growth, improving intestinal flora, and reducing the death rate

Active Publication Date: 2019-08-06
INNER MONGOLIA SCI PLUS BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the microecological preparations used to improve the water quality of farms on the market basically need to be fermented in professional system equipment before use, or, without fermentation, the dry powder of the probiotics is dissolved and directly sprinkled, which leads to the improvement of aquaculture. Water quality aquaculture farms either spend a lot of money to set up and operate professional fermentation equipment, or lose the effica

Method used

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  • Fast fermentation compound probiotic regulator for improving water quality of aquaculture farm
  • Fast fermentation compound probiotic regulator for improving water quality of aquaculture farm
  • Fast fermentation compound probiotic regulator for improving water quality of aquaculture farm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1 high-density fermented bacterial strain

[0057] Step 1, take a ring of activated Lactobacillus plantarum KT-Lp9, Lactobacillus plantarum C2, Lactobacillus plantarum LP4, Lactobacillus casei zhang and Bacillus licheniformis BL-08 respectively, and inoculate them into MRS medium respectively Cultivate five kinds of primary seed liquids, wherein the culture conditions are temperature 33-37°C, rotation speed 50-100rpm, and cultivation for 18-24h;

[0058] Step 2, transfer the primary seed liquid obtained in step 1 into the MRS medium according to the inoculation amount of 3%-10% (v / v) for secondary activation to obtain the secondary seed liquid, and the activation time is 18-24h;

[0059]In step 3, the secondary seed liquid prepared in step 2 is respectively inserted into the culture medium of the respective fermenter according to the inoculum size of 3%-10% (v / v), to obtain the final fermentation liquid of Lactobacillus plantarum KT-Lp9, plant The final fe...

Embodiment 2

[0061] Embodiment 2 Preparation of the rapid fermentation composite probiotic regulator for improving water quality in aquaculture farms

[0062] Step 1', the Lactobacillus plantarum KT-Lp9 inoculum, Lactobacillus plantarum C2 inoculum, Lactobacillus plantarum LP4, Lactobacillus casei zhang inoculum and Bacillus licheniformis BL-08 inoculum prepared in Example 2 are prepared according to weight ratio The weight of Lactobacillus plantarum KT-Lp9 inoculum: the weight of Lactobacillus plantarum C2 inoculum: the weight of Lactobacillus plantarum LP4 inoculum: the weight of Lactobacillus casei zhang inoculum: the weight=2 of Bacillus licheniformis BL-08 inoculum :1:1:3:3 for mixing;

[0063] Step 2', add nutrient-type dilution carrier to the system prepared in step 1' for compounding, and the total number of viable bacteria prepared is 1×10 8 CFU / g rapid fermentation compound probiotic regulator, wherein the nutritional dilution carrier includes sucrose, glucose, yeast powder, s...

Embodiment 3

[0065] Example 3 Lactobacillus plantarum KT-Lp9 acid resistance, bile salt resistance and its antibacterial properties experiment

[0066] The cryopreserved Lactobacillus plantarum KT-Lp9 was inoculated in the MRS liquid medium, cultured statically at 37° C. for 18 hours, and subcultured twice in this way to obtain an activated fermentation broth.

[0067] The composition of the MRS liquid medium used in this example is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL Tween 80, 0.2g sulfuric acid Add magnesium and 0.05g manganese sulfate to 1000mL distilled water, adjust the pH to 6.5, and sterilize at 121°C for 15min.

[0068] (1) Acid resistance and bile salt resistance of Lactobacillus plantarum KT-Lp9

[0069] experimental method:

[0070] 1. Simulated gastric juice treatment: Add 3.5 g / L pepsin to sterilized PBS buffer solution with pH = 2.5 (adjusted with 1 ...

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Abstract

The invention provides a fast fermentation compound probiotic regulator for improving water quality of an aquaculture farm. The fast fermentation compound probiotic regulator includes lactobacillus plantarum KT-Lp9, lactobacillus plantarum C2, lactobacillus plantarum LP4, lactobacillus casei Zhang and bacillus licheniformis BL-08. The fast fermentation compound probiotic regulator can rapidly ferment in the aquaculture farm, regulate the water quality of the aquaculture farm, does not produce secondary pollution to aquaculture water bodies, and also is a probiotic regulator for promoting growth and reducing the death and culling rate on cultured aquatic products.

Description

technical field [0001] The application belongs to the field of microbial preparations, in particular to a rapid-fermentation composite probiotic regulator for improving water quality in aquaculture farms. Background technique [0002] With the increasing development of my country's aquaculture industry, related problems have gradually emerged, including water pollution in aquaculture farms, resulting in excessive nitrite and ammonia nitrogen, poor aquaculture environmental sanitation, excessive harmful bacteria and parasites in the water, and abuse to increase the growth rate of aquatic products. Antibiotics and other drugs have caused drug residues to endanger human health and other problems. In recent years, some large-scale farms have begun to introduce probiotics to improve water quality. However, due to the single strain of the probiotics used and the lack of strict basic scientific experimental evaluation of the strains, the product function is unstable and complicated ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F103/20C12R1/125C12R1/245C12R1/10
CPCC12N1/20C02F3/34C02F2103/20Y02A40/81
Inventor 黄卫强包维臣郭建林
Owner INNER MONGOLIA SCI PLUS BIOTECH
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