Method of isolating botulinum toxin from botulinum toxin-containing solution

一种肉毒杆菌毒素、溶液的技术,应用在从含有肉毒杆菌毒素的溶液中分离肉毒杆菌毒素领域,能够解决移除等问题

Active Publication Date: 2019-08-06
MEDY TOX INC
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] According to this method, there is a problem that during the purification of the botulinum toxin protein, the neurotoxic component (i.e. a neurotoxic molecule of about 150 kDa), the medium complex (300 kDa) or the large complex (500 kDa) is dissociated and separated from the botulinum toxin bacillus toxin protein removed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of isolating botulinum toxin from botulinum toxin-containing solution
  • Method of isolating botulinum toxin from botulinum toxin-containing solution
  • Method of isolating botulinum toxin from botulinum toxin-containing solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Cultivation of Botulinum and Purification of Botulinum Toxin

[0046] 1. Cultivation of Clostridium botulinum

[0047] In this example, large amounts of botulinum type A toxin are expressed extracellularly by culturing botulinum bacteria in animal product-free medium.

[0048] The medium used was a medium containing 4% yeast extract and 0.5% glucose based on the total weight of the medium. The components of the medium were dissolved in water for injection (WFI), and the pH was adjusted to 7.2 with NaOH.

[0049] 200 ml of the culture medium thus prepared was filtered through a sterile disposable microfiltration system (0.2 μm Sartorius2, manufactured by Sartorius), and injected into the Cellbag TM (manufactured by GE healthcare, disposable fermenter bags). Then, through the sample injection port with 1ml of density 10 6 Cells / ml~10 7 Cells / ml of botulinum seed culture inoculation medium. cell bag TM is a single-use bioreactor that uses a rocking motion...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Provided is a method of isolating a botulinum toxin type A macro complex from a botulinum toxin-containing solution, the method including performing anion exchange chromatography and cation exchange chromatography.

Description

technical field [0001] Cross References to Related Applications [0002] This application claims the benefit of Korean Patent Application No. 10-2016-0127540 filed with the Korean Intellectual Property Office on October 4, 2016, the disclosure of which is incorporated herein by reference in its entirety. [0003] The present disclosure relates to methods of isolating botulinum toxin from a botulinum toxin-containing solution. Background technique [0004] From 1890 to the present, Clostridium strains that secrete neurotoxic toxins have been discovered, and the properties of the toxins secreted by these strains have been studied in the past 70 years (Schant, E.J. et al., Microbiol. Rev. 56 ; 80; 1992). [0005] The genus Clostridium includes more than 127 species, divided according to their morphology and function. Clostridium botulinum is an anaerobic and Gram-positive bacterium that produces powerful polypeptide neurotoxins that cause a neuroparalytic disease, botulism, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/18C07K14/33B01D15/36
CPCC07K14/33B01D15/362B01D15/363Y02A50/30C07K1/18C12N9/52
Inventor 丁贤壕梁起赫李彰训金学祐柳基赫洪衡杓宋晶贤孔竣燦
Owner MEDY TOX INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products