A kind of preparation method of sunflower antioxidant activity peptide
An antioxidant active peptide, sunflower technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low purity and difficult purification of sunflower peptides
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0031] The invention provides a preparation method of sunflower antioxidant active peptide, which comprises the following steps: (1) using an ethanol solution with a volume concentration of 95% to alcohol-wash defatted sunflower meal twice, and air-dry for 8-12 hours to obtain concentrated sunflower protein;
[0032] (2) Centrifuge after mixing and dissolving the concentrated sunflower protein with salt solution for 2 hours, adjust the pH value of the supernatant to acidity, and then centrifuge again to obtain protein precipitation; NaCl and 0.2-0.35% Na 2 SO 3 ;
[0033] (3) dissolving the protein precipitate in water, adjusting the pH value to 7, performing dialysis with a dialysis bag, and obtaining sunflower protein isolate after freeze-drying;
[0034] (4) utilizing protease to enzymolyze the sunflower protein isolate to obtain an enzymolyzed product; the protease includes a mixture of one or two of Flavourzyme enzyme, Alcalase enzyme and Protamex enzyme;
[0035] (5) ...
Embodiment 1
[0061] 1. Preparation of sunflower protein isolate
[0062] Prepared from salt-dissolved acid precipitation of sunflower defatted meal: washed twice with 95% ethanol alcohol (50°C, 2h, pH 4.5), meal solution ratio 1:10 (w / v), air-dried overnight at 40°C to obtain sunflower protein concentrate . With 10% NaCl, 0.25% Na 2 SO 3 Carry out salt dissolution (50°C, 2h, pH 7.0), meal solubility ratio 1:10 (w / v), centrifuge (4000 r / min, 10min), adjust the pH of the supernatant to 4.5, and centrifuge again (4000r / min min, 10min), to obtain a precipitate, dissolve the precipitate in water, adjust the pH to 7, dialyze with a dialysis bag, and freeze-dry to obtain sunflower protein isolate. The components of defatted sunflower meal and sunflower protein isolate are shown in Table 3:
[0063] Table 3 chemical composition of sunflower meal (%)
[0064]
[0065] 2. Preparation of sunflower antioxidant active peptides
[0066] (1) Preparation of sunflower protein isolate enzymatic hyd...
Embodiment 2
[0082] Structure Determination of Sunflower Antioxidant Active Peptides
[0083] (1) Peptide sequence determination
[0084] First spot 0.5 μL of the sample on the MALDI target plate, after natural drying, then spot 0.5 μL of 0.5 g / L CHCA solution (solvent, 0.1% TFA+50% ACN), and let it dry naturally at room temperature. Another point of 0.5 μ L 0.5g / L CHCA solution (unpointed sample) was used as a blank control, and the sample was subjected to mass spectrometry analysis with a 4700 tandem time-of-flight mass spectrometer (Applied Biosystems, USA), and the results were as follows: Figure 6-9 shown.
[0085](2) Four new sunflower antioxidant active peptide structures
[0086] The sequences of the four sunflower protein antioxidant active peptides isolated are shown in Table 5:
[0087] Table 5 Sequence List of Sunflower Protein Antioxidant Active Peptides
[0088]
[0089] (3) Sequence alignment of peptides
[0090] The sequences of the obtained peptides were compared...
PUM
| Property | Measurement | Unit |
|---|---|---|
| volume | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More