Method for synchronously preparing mannan oligosaccharides, peptides for feed use and dietary fiber by utilizing palm meal

Abstract

The invention belongs to the technical field of biology, and discloses a method for synchronously preparing mannan oligosaccharides, peptides for feed use and dietary fiber by utilizing palm meal. Themethod for synchronously preparing the mannan oligosaccharides, the peptides for feed use and the dietary fiber by utilizing the palm meal comprises the following steps: mixing palm meal with water,and adding a compound enzyme 1 so as to be subjected to enzymatic hydrolysis; carrying out centrifugal separation after completion of the enzymatic hydrolysis so as to obtain liquid supernatant, and drying the liquid supernatant so as to obtain mannan oligosaccharides, wherein yield of the mannan oligosaccharides from the palm meal is 80% or above and proportion of mannose to total sugar is 95% orabove; mixing solids enriched with crude proteins with water, adding a compound enzyme 2 so as to be subjected to enzymatic hydrolysis; carrying out centrifugal separation after completion of the enzymatic hydrolysis so as to obtain liquid supernatant, and drying the liquid supernatant so as to obtain peptides for feed use, wherein yield of the peptides from the proteins of the palm meal is 90% or above and content of small peptides is 80% or above; and then, adding a compound enzyme 3 and a compound microbial agent into solids containing crude fiber, and carrying out fermentation so as to obtain dietary fiber for feed use, wherein content of soluble fiber is 30-50%, content of mannan oligosaccharides is 10-20%, content of lactic acid is 8% or above, total number of lactic acid bacteria is 5 x 10<9> CFU/g and total number of yeast is 1 x 10<9> CFU/g. Thus, a high-quality feed additive is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for simultaneously preparing mannan oligosaccharides, polypeptides for feed and dietary fiber by using palm dregs. Background technique [0002] Palm meal is a by-product of oil extraction from palm kernel fruit. It is brown in color and has a chocolate smell. The content of crude protein and crude fat in palm kernel meal is higher than that of conventional feed such as corn and bran, and it is a feed material with high cost performance. Palm kernel meal is rich in phosphorus, copper, manganese, potassium and other minerals, as well as a variety of vitamins and amino acids. It can be used as energy feed mixed with other feeds, and has good application potential. [0003] However, there are still limitations in the actual use of palm meal: first, due to the influence of processing conditions, its taste is slightly bitter, and some shell-like debris is relatively ...

AI Technical Summary

Problems solved by technology

[0003] However, there are still limitations in the actual use of palm meal: first, due to the influence of processing conditions, its taste is slightly bitter, and some shell-like debris is relatively hard in texture, which affects the consumption of poultry and livestock

Second, the protein content of palm meal is low, at 15%-20%, and it is difficult to replace it with protein raw materials such as soybean meal, cotton meal, and rapeseed meal

Third, palm meal contains more non-starch polysaccharides, including 78% linear mannan, 3% arabinoxylan, 3% glucuronic acid xylan, 12% cellulose, especially mannan Sugar has a strong anti-nutritional effect, which will reduce the digestibility of poultry and pigs; and inhibit the se