Recombination baculovirus for expressing Africa swine fever CD2V protein in SF9 cell

A technology of recombinant baculovirus and African swine fever virus, which is applied in the direction of double-stranded DNA virus, virus, viral peptide, etc., can solve the problem of vaccine without African swine fever, etc., and achieve the effect of high protein expression and easy purification
CN110157737AInactive Publication Date: 2019-08-23YEBIO BIOENG OF QINGDAO
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YEBIO BIOENG OF QINGDAO
Publication Date
2019-08-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a recombination baculovirus for expressing Africa swine fever CD2V protein in an SF9 cell. The CD2V protein can be recombined and expressed in the SF9 cell; the amino acid sequence of the CD2V protein is shown in SEQ ID NO:4; the sequence of the nucleotide fragment is shown in SEQ ID NO:3. The recombination baculovirus is used for preparing the Africa swine fever CD2V protein in an insect cell. The insect cell Sf9 is used for recombining and expressing the Africa swine fever CD2V protein, the protein expression amount is high, purification is easy, the recombination baculovirus is used for preparing and identifying a diagnosis product, and a solid foundation is laid for producing Africa swine fever subunit vaccines and diagnosis reagents.
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Description

technical field

[0001] The invention belongs to the technical field of veterinary biological products, in particular to a recombinant baculovirus expressing African swine fever CD2V protein in SF9 cells. Background technique

[0002] African swine fever (African Swine fever, East African Swine fever, ASF) is an acute, febrile and highly contagious viral disease caused by a viral virus, characterized by a short course of disease, but a mortality rate of up to 100%. , clinical manifestations of fever, skin cyanosis, lymph nodes, kidneys, gastrointestinal mucosal bleeding. The disease was first reported in Kenya in 1909 and has always existed in sub-Saharan African countries. It spread to Western Europe and Latin American countries in 1957, and most of them were extinguished in time. However, there are still cases in Portugal, southwestern Spain and Sardinia in Italy. Popularity. Since 2007, African swine fever has occurred, spread, and spread in many countries around the wor...

Claims

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