Polymorphic molecular marker for SINE transposon inside GHR gene related to pig back fat thickness, detection method and application

Abstract

The invention discloses a polymorphic molecular marker for a SINE transposon inside a GHR gene related to pig back fat thickness, a detection method and application. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:1, the molecular marker comes from a GHR gene, an insertion polymorphic locus of a reverse SINE transposon exists on each of 200-493 loci of the sequence, the lociare shown as three gene types of SINE+ / +, SINE+ / - or SINE- / -, the body back fat thickness of the homozygous insertion gene type (SINE+ / +) is remarkably smaller than that of the body of the homozygousnon-insertion gene type (SINE- / -). The polymorphic molecular marker for the SINE transposon inside the GHR gene can be used for early selection of family breeding, and the molecular basis can be supplied in apolegamy. The powerful auxiliary effect is supplied to breeding of the back fat character, the breeding process is accelerated, and the breeding cost is lowered. The polymorphic molecular marker for a SINE transposon inside the GHR gene supplies a novel method for screening the pig back fat thickness.

Description

technical field [0001] The invention belongs to the field of animal genetics and breeding, and also relates to the fields of bioinformatics and molecular biology, in particular to a SINE transposon polymorphic molecular marker in the GHR gene related to pig backfat thickness, its acquisition method and application. Background technique [0002] In the porcine genome, retrotransposons are the main components of the genome, accounting for 37.10% of the porcine genome and 91.49% of the repetitive sequences. Due to their high coverage in the genome and their ability to move, transposons are thought to be important contributors to genome structural variation and may affect the activity of host genes at multiple levels. More than 100 retrotransposon-mediated insertions leading to human genetic diseases have been reported (Hancks and Kazazian, Roles for Retrotransposon Insertions in Human Disease. 2016). Many phenotypic changes caused by transposon insertions have been observed in...

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Problems solved by technology

However, there is no report on whether there are transposon insertion polymorphisms in the GHR gene and the impact on backfat thickness. found an insertion polymorphism of a SINE transposon in the large white pig population, and found that this molecular marker has a significant correlation with the backfat thickness of pigs

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