Quick subtype detection method for HPV in urine

Abstract

The invention relates to a quick subtype detection method for HPV in urine. The quick subtype detection method sequentially comprises the following steps of (1) centrifuging urine, discarding supernatant, taking precipitate cells, cleaning the taken precipitate cells with PBS, adding lysate, performing heating, then performing high speed centrifugation once again, taking supernatant, and performing subsequent qPCR; (2) performing qPCR amplification on a qPCR system consisting of a buffer solution, multiple DNA polymerases, mixed primers, mixed probes, the supernatant in the step (1) and water;and (3) detecting amplification products. According to the quick subtype detection method for HPV in urine provided by the invention, quick qPCR is adopted for detecting the HPV, and subtype is performed; according to the method, DNA extraction is not needed, so that cost can be saved, and the operation can be simplified; and when the detection is performed by the method, the detection can be performed only when urine samples are collected, and collecting urine samples is simpler and easier to accept compared with a traditional method of collecting vaginal secretion.

Description

technical field [0001] The invention belongs to the technical field of gene diagnosis, and in particular relates to a urine HPV rapid typing detection method. Background technique [0002] Cervical cancer is one of the common female malignant tumors, its incidence is second only to breast cancer, ranking second in gynecological tumors. Scientific research has confirmed that persistent infection of high-risk papillomavirus (hrHPV) is the main cause of cervical cancer, and HPV-negative women have an extremely low risk of cervical cancer. However, most women who test positive for HPV do not develop precancer or cancer, and only a minority of women with HPV infection are at risk of developing cervical precancer. [0003] HPV detection can mainly check whether you are infected with HPV through staining microscopy, HPV DNA detection or serological tests. The mainstream detection is still through cervical cell sampling for total DNA extraction, followed by HPV qPCR typing detecti...

AI Technical Summary

Problems solved by technology

This method is relatively direct, simple and fast, but there are still some problems in its promotion: First, although cervical cell sampling is a non-invasive test, the requirements for sampling are relatively high, and it is easy to cause slight damage to the cervical and vaginal epithelial tissues, and most of them need to be operated in a hospital. ; In addition, most women still resist cervical cell sampling due to privacy concerns

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