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Hydrogen peroxide electrochemical sensor electrode, preparation method and sensor

A sensor electrode, hydrogen peroxide technology, applied in the field of nano biomaterials and electrochemical biosensors, to achieve the effects of simple process, thickness control, and simple process

Inactive Publication Date: 2019-09-10
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these documents did not further indicate which kind of substance the electrode with this structure would have specific detection performance, nor did it point out which kind of substance the electrode would have specific detection performance after further modification.

Method used

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  • Hydrogen peroxide electrochemical sensor electrode, preparation method and sensor
  • Hydrogen peroxide electrochemical sensor electrode, preparation method and sensor
  • Hydrogen peroxide electrochemical sensor electrode, preparation method and sensor

Examples

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Embodiment 1

[0138] A 10nm-thick Cr was evaporated on a clean silicon wafer as a metal transition layer.

[0139] Using an alloy formed by infinite mutual solubility of gold and silver (the atomic ratio of gold and silver is 65:35) as a target, sputtering on the Cr transition layer for 5 min to obtain a 200nm thick alloy film, after that, use concentrated nitric acid (69% by mass) to remove alloy for 6 hours to obtain a porous structure with a pore diameter of 30 nm. The gold film obtained after dealloying has a nanoporous structure, and the Ra value is 30nm.

[0140] A three-electrode system is adopted, that is, the gold film is used as the working electrode, the Ag / AgCl is used as the reference electrode, and the Pt wire is used as the counter electrode. 3+ and Fe(CN) 6 3+ , K + And the mixed solution of HCl is used as the electrolyte for electrochemical deposition, and the electrochemical deposition is carried out for 120s at a constant deposition voltage of 0.4V (relative to the Ag...

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Abstract

The invention relates to a hydrogen peroxide electrochemical sensor electrode, a preparation method and a sensor. The sensor comprises a hydrogen peroxide sensor electrode, wherein the hydrogen peroxide sensor electrode comprises a substrate, a gold film on the substrate and a modifying layer formed on the gold film; the gold film is of a porous structure, and is 20 to 50nm in mean pore size; andthe modifying layer is made of Prussian blue. The hydrogen peroxide sensor has a hydrogen peroxide detection limit of 0.25[mu]m or less, and has linear response within a range of 1 to 10000[mu]m.

Description

technical field [0001] The invention belongs to the field of nanobiological materials and electrochemical biosensors, and in particular relates to a hydrogen peroxide sensor electrode based on a nanoporous gold film, a hydrogen peroxide sensor based thereon, and their preparation. Background technique [0002] Hydrogen peroxide is not only one of the most important components ubiquitous in natural ecosystems, but also an intermediate substance in many industrial processes, and a by-product of various enzyme reactions in many organisms. [0003] Hydrogen peroxide (H 2 o 2 ) participation or production. Especially in organisms, the reaction by-products of many oxidases contain H 2 o 2 . For many hormones and metabolites in organisms, such as blood sugar, lactic acid, cholesterol, alcohol, etc., the quantitative detection mode is usually based on the enzyme-catalyzed substrate and H 2 o 2 The oxidation-reduction reaction produces light and electrical signals, and then de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/307G01N27/308G01N27/327
Inventor 鲁思渊黄金磊冯雪
Owner NINGBO UNIV
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