Method for utilizing distiller's grains to extract dietary fibers
A technology for dietary fiber and distiller's grains, applied in the fields of application, food science, food ingredients, etc., can solve the problems of high moisture content, perishability, negative impact on the surrounding environment of distiller's grains, etc., to achieve high extraction efficiency, reduce pollution, improve utilization value and The effect of utilization
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Embodiment 1
[0014] A method for extracting dietary fiber from distiller's grains, comprising the following steps:
[0015] S1. Take the distiller's grains, add water to grind, adjust the pH to 4, add an appropriate amount of protease for enzymolysis for 5 hours, and obtain an enzymatic hydrolysis product;
[0016] The mass ratio of the distiller's grains to the water is 1:10, and the particle size after refining is 60 mesh; the added amount of the proteolytic enzyme is 4% of the weight of the distiller's grains; the enzymatic hydrolysis temperature is 45°C;
[0017] S2. Ultrasonic extraction of the enzymatic hydrolyzate for 50 minutes, with an ultrasonic power of 300 W, and filtering to remove the precipitate to obtain an ultrasonic extract;
[0018] S3. Adjust the pH of the ultrasonic extract to neutral, concentrate to 20% of the original volume, add an appropriate amount of absolute ethanol, let stand for precipitation for 1 hour, filter and dry to obtain dietary fiber.
[0019] The ad...
Embodiment 2
[0021] A method for extracting dietary fiber from distiller's grains, comprising the following steps:
[0022] S1. Take the distiller's grains, add water to grind, adjust the pH to 5, and add an appropriate amount of protease for enzymolysis for 6 hours to obtain an enzymatic hydrolysis product;
[0023] The mass ratio of the distiller's grains to the water is 1:13, and the particle size after refining is 80 mesh; the added amount of the hydrolytic protease is 5% of the weight of the distiller's grains; the enzymatic hydrolysis temperature is 50°C;
[0024] S2. Ultrasonic extraction of the enzymatic hydrolyzate for 60 min, with an ultrasonic power of 400 W, and filtering to remove the precipitate to obtain an ultrasonic extract;
[0025] S3. Adjust the pH of the ultrasonic extract to neutral, concentrate it to 25% of the original volume, add an appropriate amount of absolute ethanol, let it settle for 1.5 hours, filter, and dry to obtain dietary fiber.
[0026] The added amou...
Embodiment 3
[0028] A method for extracting dietary fiber from distiller's grains, comprising the following steps:
[0029] S1. Take the distiller's grains, add water to grind, adjust the pH to 6, add an appropriate amount of proteolytic enzyme to hydrolyze for 7 hours, and obtain an enzymatic hydrolysis product;
[0030] The mass ratio of the distiller's grains to the water is 1:15, and the particle size after refining is 100 mesh; the added amount of the hydrolytic protease is 6% of the weight of the distiller's grains; the enzymatic hydrolysis temperature is 55°C;
[0031] S2. Ultrasonic extraction of the enzymatic hydrolyzate for 70 minutes, with an ultrasonic power of 500 W, and filtering to remove the precipitate to obtain an ultrasonic extract;
[0032] S3. Adjust the pH of the ultrasonic extract to neutral, concentrate it to 30% of the original volume, add an appropriate amount of absolute ethanol, let it settle for 2 hours, filter, and dry to obtain dietary fiber.
[0033] The ad...
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