A method for making schistosome egg specimens by using improved Kato thick smear
A technology for making schistosomiasis eggs and smears, which is applied in the preparation, sampling, and instrumentation of test samples. It can solve the problems of morphological changes of schistosomiasis eggs and inaccurate specimen shapes, and achieve the effect of easy storage, easy observation, and better preservation
Active Publication Date: 2020-06-02
四川省疾病预防控制中心 +1
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Problems solved by technology
[0006] When using the above method, since the scraper is used to scrape the nylon silk and the feces containing the schistosome ovum to pass through the 100 mesh / inch nylon silk, this process will produce extrusion and filtration of the schistosome ovum, resulting in worms. The shape of the egg changes, making the shape of the prepared specimen inaccurate
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Embodiment 1
[0030] Embodiment 1: obtaining schistosome eggs. The process is as follows:
[0031] Take the liver of rabbits infected with schistosome-positive snail cercaria for 42 days, remove the connective tissue and blood vessels in the liver, and divide the remaining liver into several small pieces; pour the small pieces of liver tissue into a high-speed tissue masher, add ice Smash the saline for 3 times, 2 minutes each time, 2 minutes apart, pass the crushed liver residues through 40, 60, 80, and 120 mesh / inch sampling sieves, wash them repeatedly with ice saline, and then collect the sediment in a triangular measuring cup , and placed in the refrigerator for natural precipitation; then the supernatant was removed, and the sediment was centrifuged and precipitated three times to collect pure schistosome eggs, which were stored in a refrigerator with 1.2% normal saline for later use.
[0032] The feces acquisition process is as follows:
[0033] Carry out parasitic disease investig...
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The invention relates to a method for preparing a schistosome egg specimen by using Kato-Katz. The method comprises the following steps: taking human feces without parasitic infection, scraping fine fecal residues by using 100 mesh / h nylon silk, adding 50-500 schistosome eggs into the fine dung residues, and stirring evenly; placing a Kato-Katz quantification plate on a glass slide, filling quantification micropores of the quantification plate with the fine fecal residues containing the schistosome eggs, removing the quantification plate to retain the fine fecal residues in the micropores on the glass slide, then covering the glass slide with transparent glass paper, and finally extruding the fine fecal residues to scatter and level the same; and obtaining a Kato-Katz specimen. By adopting the method provided by the invention, in the process of preparing the Kato-Katz schistosome egg specimen, the extrusion of a scraping sheet on the schistosome eggs and the extrusion of the nylon silk on the schistosome eggs are avoided, so that the complete forms of the schistosome eggs can be kept, and very good specimens are provided for microscopic examination schistosome egg technical training.
Description
technical field [0001] The invention relates to a method for preparing schistosome egg specimens by using the improved Kato thick smear. Background technique [0002] Schistosomiasis is prevalent in 12 provinces (cities, districts) in southern my country. There are 100 million people threatened by schistosomiasis nationwide, and more than 12 million patients with schistosomiasis in history. Schistosomiasis seriously endangers people's physical and mental health and hinders social and economic development. It has always been a key prevention and control in my country. of serious diseases. The etiological detection methods of schistosomiasis are the gold standard for the diagnosis of schistosomiasis, mainly including schistosomiasis miracidium hatching method, modified Kato-Katz method, and liver and rectal biopsy examination. Among them, liver and rectal biopsy examinations are seldom carried out due to the large damage; the incubation method is cumbersome to operate, and the...
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IPC IPC(8): G01N1/28
CPCG01N1/2806G01N1/2813G01N2001/2893
Inventor 吴子松陈陵李荣智杨羽毛勇徐亮姚琴蒙先洪范建儒唐猛刘阳钟波
Owner 四川省疾病预防控制中心