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Preparation method of polyethyleneimine/chiral peptide modified glassy carbon electrode and application thereof in tryptophan enantiomer selective recognition

A technology of tryptophan enantiomer and polyethyleneimine, which is applied in electrochemical analysis and biological fields, can solve the problems of low sensitivity, complicated operation and high analysis cost, and achieves the effects of improved recognition effect and cheap preparation method.

Active Publication Date: 2019-09-17
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some existing spectroscopic analysis methods have shortcomings such as low sensitivity, complicated operation, and high analysis cost, so it is very important to find an analytical method with good prospects

Method used

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  • Preparation method of polyethyleneimine/chiral peptide modified glassy carbon electrode and application thereof in tryptophan enantiomer selective recognition
  • Preparation method of polyethyleneimine/chiral peptide modified glassy carbon electrode and application thereof in tryptophan enantiomer selective recognition
  • Preparation method of polyethyleneimine/chiral peptide modified glassy carbon electrode and application thereof in tryptophan enantiomer selective recognition

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Experimental program
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Effect test

Embodiment 1

[0023] Preparation of PEI / D-BGAc for SEM Characterization

[0024] a. Weigh 50mg of PEI and dissolve it in 50mL of deionized water to prepare a 1mg / mL PEI solution.

[0025] b. Weigh 25 mg of D-BGAc chiral peptide, refer to [1] for the method of preparing D-BGAc, and disperse it in 50 mL of deionized water to prepare a 0.5 mg / mL D-BGAc solution.

[0026] c. Clean the silicon wafer and dry it with nitrogen, place it in 1 mg / mL PEI solution, and soak it for 15 minutes to obtain a PEI-modified silicon wafer.

[0027] d. Wash the PEI-modified silicon wafer prepared in step c with deionized water, dry it with nitrogen, and immerse it in a D-BGAc solution containing 0.5 mg / mL, soak for 15 minutes, take it out and dry it at room temperature to obtain PEI / D-BGAc membrane.

Embodiment 2

[0029] The steps for preparing PEI-modified electrodes, D-BGAc-modified electrodes and PEI / D-BGAc-modified glassy carbon electrodes for electrochemical recognition of tryptophan enantiomers are as follows:

[0030] Preparation of PEI-modified electrode: prepare 4 mL of 1 mg / mL PEI solution, immerse the glassy carbon electrode in the PEI solution for 30 minutes, and obtain a PEI-modified glassy carbon electrode.

[0031] Preparation of D-BGAc modified electrode: prepare 4 mL of 0.5 mg / mL D-BGAc solution, immerse the glassy carbon electrode in the D-BGAc solution for 30 min to obtain a D-BGAc modified glassy carbon electrode.

[0032] Preparation of PEI / D-BGAc modified electrode: Wash the prepared PEI modified glassy carbon electrode with deionized water, dry it with nitrogen gas and immerse it in a D-BGAc solution containing 0.5 mg / mL for 15 minutes to obtain PEI / D-BGAc Modified glassy carbon electrodes.

[0033] The PEI modified electrode prepared above, D-BGAc modified elect...

Embodiment 3

[0039] The steps for preparing the PEI / D-BGAc modified electrode are as in Example 2. The prepared modified electrode was used as the working electrode, the Ag / AgCl electrode was used as the reference electrode, and the platinum wire electrode was used as the counter electrode. The three-electrode system was respectively immersed in 10 mL containing different mixing ratios of L-tryptophan and D-tryptophan in the PBS solution. The DPV test was carried out in the potential window of 0.4-1V, and the graph of the change of current with the content of L-tryptophan was obtained. Such as Figure 6 Shown: The current has a linear relationship with the L-tryptophan content.

[0040] [1]Li C, Jin X, Zhao T, Zhou J, Duan P.Optically active quantum dots withininduced circularly polarized luminescence in amphiphilic peptide dendronhydrogel. Nanoscale Advances 2019,1,508-512. .

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Abstract

The present invention relates to a preparation method of a polyethyleneimine / chiral peptide modified glassy carbon electrode and an application thereof in tryptophan enantiomer selective recognition. The method comprises following steps: preparing a polyethyleneimine modified glassy carbon electrode, and immersing the polyethyleneimine modified glassy carbon electrode in a chiral peptide (BGAc) solution for further modification, to obtain the polyethyleneimine / chiral peptide modified glassy carbon electrode, for electrochemical identification of a tryptophan enantiomer. The method has beneficial effects that: the method has simple preparation process of the polyethyleneimine / chiral peptide modified electrode, is environmentally friendly, low in costs and obtains the modified electrode having obvious recognition effect on the tryptophan enantiomer.

Description

technical field [0001] The invention relates to the preparation of a polyethyleneimine / chiral peptide modified glassy carbon electrode and its application to the selective recognition of tryptophan enantiomers, belonging to the fields of electrochemical analysis and biotechnology. Background technique [0002] Amino acids provide an important material basis for the metabolism, life activities, growth and development of organisms, and can be used as important biomarkers for various metabolic diseases. There are D-type and L-type configurations of amino acid isomers. In practical applications, one enantiomer often has pharmacological effects on the human body, while the other enantiomer is ineffective or has serious side effects or even toxicity. Therefore, the research on the recognition of chiral amino acids is particularly important and meaningful in the fields of pharmacy, biological analysis, drug screening, and analytical separation. Some existing spectroscopic analysis...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3275G01N27/3277G01N27/48
Inventor 孙一新何家辉张嵘盛扬黄经纬徐德峰
Owner CHANGZHOU UNIV
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