Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor

A deubiquitinating enzyme and interferon technology, applied in the field of biomedicine, can solve the problems of few research reports on 3C-like proteases and unclear biological functions

Inactive Publication Date: 2019-09-20
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] Studies in the prior art have found that papain-like proteases participate in a series of important functions of innate immune response, but there are few reports on 3C-like proteases, and their biological functions are still unclear

Method used

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  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor
  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor
  • Application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as deubiquitinating enzyme and interferon inhibitor

Examples

Experimental program
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Embodiment

[0043] This example clarifies the mechanism of 3CLpro regulating host antiviral innate immune response from various aspects and perspectives, and clarifies the use of MERS-CoV 3CLpro as a deubiquitinating enzyme and interferon inhibitor.

[0044] 1 method

[0045] 1.1 Construction of MERS-CoV / SARS-CoV / NL63-CoV 3CLpro and its mutants

[0046] In order to study the structure and biological characteristics of MERS-CoV / SARS-CoV / NL63-CoV, the present invention obtains the MERS-CoV / SARS-CoV / NL63-CoV 3CLpro sequence by comparing and analyzing the genome sequence, and at its C-terminal Connect the V5 sequence, artificially synthesized by GenScript. Using MERS-CoV / SARS-CoV / NL63-CoV 3CLpro as a template, mutants were obtained by site-directed mutagenesis.

[0047] 1.1.1 Amino acid sequence of MERS-CoV / SARS-CoV / NL63-CoV 3CLpro:

[0048] Amino acid sequence of MERS-CoV 3CLpro: shown in SEQ ID NO:1.

[0049] SARS-CoV 3CLpro amino acid sequence: shown in SEQ ID NO:2.

[0050] NL63-CoV ...

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Abstract

The invention relates to the field of biomedicine, in particular to application of MERS-CoV 3CLpro (Middle East respiratory syndrome coronavirus 3C-like protease) as a deubiquitinating enzyme and interferon inhibitor. The invention provides a novel deubiquitinating enzyme and interferon inhibitor; the new understanding of the functionality of human coronavirus proteinase can be expanded; theoretical basis can be provided for the research on coronavirus and host antiviral natural immunization and the research on antiviral drugs using viral proteinase as a target.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the use of MERS-CoV 3CLpro as a deubiquitinase and interferon inhibitor. Background technique [0002] Coronavirus (Coronavirus, CoV) is a typical member of the genus Coronavirus in the Coronaviridae family. It is a single-stranded positive-sense RNA virus that can infect vertebrates and humans and cause various diseases such as respiratory tract infection and gastroenteritis. The new human coronavirus-Middle East Resperatory Syndrome coronavirus (MERS-CoV) was first isolated from a 60-year-old Saudi patient who died of respiratory infection and renal failure at the end of 2012. According to the statistics of the World Health Organization, from September 2012 to April 2019, a total of 2,428 confirmed cases of MERS have been found in the world, of which 838 died, with a fatality rate as high as 35%, which seriously threatens human life and health (WHO website) . In recent year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48G01N33/573
CPCC12N9/485C12Y304/19012G01N33/573G01N2333/948G01N2500/04G01N2500/10
Inventor 邢雅玲陈忠斌解晴陈晓娟
Owner ACADEMY OF MILITARY MEDICAL SCI
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