Respiratory virus detection kit and method

Abstract

The invention discloses a respiratory virus detection kit. The detection kit is used for detecting ORF1ab area and N region of SARS-COV-2 viruses; the detection kit includes the following primers andprobes: amplification primers ORF1ab-F, ORF1ab-R and ORF1ab-probe used for amplifying the ORF1ab area; and amplification primers N-F, N-R and N-probe used for amplifying the N region. Through the adoption of areal-time fluorescence quantitative PCR technology, the detection kit can be used for rapidly detection whether a to-be-detected sample is a new coronavirus positive sample. Detection resultsare accurate by using the detection kit; and therefore, the detection kit has important reference significance of rapid diagnosis on the infection status of SARS-COV-2 human coronavirus.

Description

technical field

[0001] This patent relates to a virus detection kit for clinical testing, which uses probe Taqman real-time fluorescent quantitative PCR technology for nucleic acid detection of SARS-COV-2 virus. The kit adopts double PCR method and uses human internal standard RNase P to effectively control the whole process and reduce false negatives. Background technique

[0002] Coronaviruses (Covs) are important pathogens infecting humans and animals, often associated with respiratory and gastrointestinal infections. Covs are balloon-enveloped viruses with a diameter of 100-160 nanometers. Each virus contains a 27-32kb positive-sense single-stranded RNA genome. The viral envelope contains three distinct proteins: membrane protein (m), envelope protein (e), and spike protein (s). The M protein binds to the nucleocapsid and participates in virus assembly and budding, and the E protein participates in virus formation and release. The S protein forms homotrimers that rec...