An anthocyanin producing strain cj6 and its application

An anthocyanin, CJ6 technology, applied in fungi, microorganisms, microorganisms and other directions, can solve problems such as difficulty in annual production, and achieve the effects of low cost, simple process and short production cycle

Active Publication Date: 2020-09-08
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to achieve annual production due to the influence of resources to produce anthocyanins with plants

Method used

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  • An anthocyanin producing strain cj6 and its application
  • An anthocyanin producing strain cj6 and its application
  • An anthocyanin producing strain cj6 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Example 1 Bacterial Strain CJ6

[0038] 1. Strain isolation and purification

[0039] (1) Collect multiple soils from different regions, grind the soil samples into powder, and then dilute them to 10 with normal saline according to the gradient of 1:10, 1:100, 1:1000, 1:10000; 1:100000 -1 -10 -8 solution. Take 100-200 ul of the diluted solution, spread it on beef extract peptone solid medium, Martin's solid medium, and potato dextrose (PDA) solid medium respectively, and isolate bacterial strains. Place at 25-37°C and culture for 3-7 days. Each dilution was set up in triplicate.

[0040] (2) Select colored colonies in the above-mentioned plate, and streak on the above-mentioned corresponding separation medium for separation and purification. Repeat this step 2-3 times until a single colony is obtained, and store it on the slant of the test tube. The medium formulation used is as follows:

[0041] Beef extract peptone medium: beef e...

Embodiment 2

[0073] Embodiment 2 Bacterial strain CJ6 and fermented broth bacteriostasis test thereof

[0074] Based on the antibacterial test, it was found that the strain CJ6 and its fermentation broth had no effect on the growth of the indicator bacteria, that is, no biological toxicity and environmental friendliness. The experimental method is as follows:

[0075] (1) Cultivation of strain CJ6 and preparation of test samples

[0076] Inoculate CJ6 into Martin's liquid medium or potato dextrose liquid medium, culture for 2-6 days, draw 4mL-10ml of fermentation broth, and centrifuge at 800R for 10min to obtain the supernatant of the fermentation broth (which contains anthocyanins) and precipitate bacteria, spare.

[0077] (2) configuration of antibiotic control group solution

[0078] Weigh 20.0mg of aureomycin antibiotic, place in 100mL distilled water, and make 200mg / L aureomycin solution for later use.

[0079] (3) Activation culture of indicator bacteria

[0080] Select Micrococ...

Embodiment 3

[0085] Embodiment 3 bacterial strain CJ6 grows, metabolizes anthocyanin and application

[0086] 1. Effects of different initial pH values ​​on strain growth and anthocyanin synthesis

[0087] Configure Martin's and potato dextrose liquid medium, adjust the pH value of the medium to 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12 with hydrochloric acid or sodium hydroxide respectively, and then subpackage, Set 3 parallel mediums for each pH value, sterilize at 115-121°C for 15-20 minutes, cool down, inoculate the seed solution of strain CJ6 according to the inoculum amount of 1-10%, and inoculate the seed solution of strain CJ6 at the 2nd, 4th, 6th, and 8. Determination of OD in 10 days 520 value, and observe the color change. The results showed that the strain CJ6 could grow in the medium with a pH value of 3-10, and could metabolize and produce anthocyanin in a medium with a pH value of 3-9.

[0088] 2. Effects of different media and different carbon sources on the growth and antho...

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Abstract

The invention relates to an anthocyanin-producing strain CJ6 and its application, belonging to the field of microbial resources and technology. The strain CJ6 is identified as Fusarium proliferatum, and the preservation number is CGMCC NO.14793. Strain CJ6 is suitable for a wide range of growth and is easy to cultivate. It can synthesize anthocyanins from agricultural solid waste, grain raw materials and various carbon sources. It has low production cost, short cycle and environmental friendliness. Here comes a new resource with broad application prospects.

Description

technical field [0001] The invention relates to an anthocyanin-producing strain CJ6 and its application, belonging to the field of microbial resources and microbial technology. Background technique [0002] Anthocyanins belong to flavonoid compounds, which have physiological functions such as scavenging free radicals, anti-cancer, anti-oxidation, anti-mutation, anti-radiation, preventing cardiovascular and cerebrovascular diseases, improving skin elasticity, improving vision, improving sleep, and protecting the liver. They are widely used In medicine, cosmetics, health products, food and other fields. [0003] Plant anthocyanins are high in blueberries, mulberries, grapes and other plants, and are one of the main pigments that make up the color of petals and fruits. Its color shows different colors of red, blue and purple with the change of pH value, and has an absorption peak in the visible light region of 500-550nm. [0004] Plant growth is greatly affected by environmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12P17/06C12R1/77
CPCC12N1/02C12P17/06C12N1/145C12R2001/77
Inventor 李艳菊陈静吕长银马一栋王可欣杨正礼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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