Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus siamensis, preparation method and usage thereof

A technology of Bacillus simum, which is used in the field of microorganisms, can solve the problems of difficult storage and transportation, complicated preparation procedures, and complicated procedures, and achieve the effects of great disease resistance potential, simple preparation procedures, and huge research and development potential

Active Publication Date: 2019-10-08
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
View PDF12 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When used as a biocontrol agent, the preparation process of the dosage form is complicated, and most of them are mixed with multiple strains and biochemical materials. Difficult to store and transport, inconvenient to use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus siamensis, preparation method and usage thereof
  • Bacillus siamensis, preparation method and usage thereof
  • Bacillus siamensis, preparation method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Isolation and extraction of bacterial strain

[0028] Marine materials: The marine materials are deep-sea sediments excavated from the South China Sea-3000m scientific research, donated by Associate Researcher Tang Xixiang of the Key Laboratory of Marine Biogenetic Resources of the Third Institute of Oceanography, State Oceanic Administration.

[0029] Seven common crop pathogenic fungi: all purchased from the China Agricultural Microbiological Culture Collection Center, specifically Phytophthora citricola strain number 36282 (Phytophthora citricola), strawberry gray mold Botrytis cinerea strain number 37271 (Botrytis cinerea Pers. ex Fr.), Fusarium oxysporum strain No. 36933 (Fusarium oxysporum), Sclerotium rolfsii strain No. 37946 (Sclerotium rolfsii), and Alternaria oxysporum strain No. 37286 (Alternaria tenuissima), Rhizoctonia solani strain No. 37289 (Rhizoctonia solani), and Alternaria brassicicola strain No. 37290 (Alternaria brassicicola).

[0030...

Embodiment 2

[0052] Embodiment 2: Cabbage test effect verification

[0053] The powder obtained in Example 1 was diluted with water at a volume ratio of 1:50, 1:100, 1:200, 1:400, and 1:800, (results showed that 1:200 and 1:50, 1:100 effect difference Not significant, select 1:200 for saving drug cost) to be prepared into aqueous solution, in the plant disease of cabbage (the plant disease of mixing 7 kinds of pathogenic bacteria: 7 kinds of pathogenic bacteria are cultivated 7d on PDA medium, use 5mL0.02% of Tween 20 solution washes off the spores of the bacteria on the medium, mixes them to obtain a spore suspension of the combined pathogen, dilutes it with normal saline at a ratio of 1:100, and sprays the spore suspension on the leaves at the base of the cabbage). Before the high-incidence period (March), the plant leaves and roots of the cabbage were sprayed. The medication cycle is once a week, a total of 3 times before and after. The amount of spraying each time is better when the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Bacillus siamensis, a preparation method and the use thereof. The Bacillus siamensis is the Bacillus siamensis sp HY-075, and is preserved in Guangdong microbial culture preservation center, and the preservation number is GDMCC 60680. The Bacillus siamensis has the use of prevention and curing vegetable plant diseases.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Sim bacillus and its application. Background technique [0002] The currently patented terrestrial soil or plant-endogenous Bacillus siamensis are all terrestrial soil and plant endogenous, and its use is to be developed into a fertilizer or a biocontrol agent for preventing and controlling a single disease. When used as a biocontrol agent, the preparation process of the dosage form is complicated, and most of them are used in combination with multiple strains and biochemical materials. It is not easy to store and transport, and it is inconvenient to use. [0003] At the same time, with the gradual reduction of available terrestrial biological resources, people turn their attention to other ecosystems, and the ocean, which accounts for 71% of the earth's resources, has become the first choice. Marine organisms account for about 80% of the total population of organisms on the ear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 林志楷林文珍卢秋华郭迟鸣
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com