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Fish environment DNA dynamic water fate characteristic testing method and device

A characteristic test, fish technology, applied in the direction of biochemical cleaning equipment, biochemical equipment and methods, enzymology/microbiology equipment, etc., can solve the problems of testing environmental DNA dynamic and water fate characteristics, etc.

Active Publication Date: 2019-10-08
CHANGJIANG RIVER SCI RES INST CHANGJIANG WATER RESOURCES COMMISSION
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  • Application Information

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Problems solved by technology

Studies have found that there is a correlation between the concentration of environmental DNA and the biological characteristics of fish and the environmental characteristics of water bodies, but trying to apply it to predict and evaluate the distribution of fish and their reproductive habitats, life history behavior, biomass, etc., still needs to solve how to test environmental DNA The bottleneck problem of dynamic water fate characteristics

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  • Fish environment DNA dynamic water fate characteristic testing method and device
  • Fish environment DNA dynamic water fate characteristic testing method and device
  • Fish environment DNA dynamic water fate characteristic testing method and device

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Embodiment Construction

[0048]The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, but they do not constitute a limitation of the present invention, they are only examples, and at the same time, it is convenient for those skilled in the art to understand the content and advantages of the present invention more clearly.

[0049] Such as figure 1 As shown, the embodiment of the present invention provides a method for testing the dynamic and water fate characteristics of fish environmental DNA, which is characterized in that it comprises the following steps:

[0050] Step (1): collecting live biological samples of target test fish;

[0051] Specifically, live biological samples of target test fish are collected from the wild or breeding grounds, including adult fish, juvenile fish, fish eggs, etc.; at the same time, the basic biological characteristics of fish are tested, including weight, size, and developmental stage. Accl...

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Abstract

The invention provides fish environment DNA dynamic water fate characteristic testing method and device, and relates to the field of water environment and water ecological protection. The fish environment DNA dynamic water fate characteristic testing method comprises the steps that (1), living fish biological samples of a target test are collected; (2), an experiment on fish environment DNA detachment characteristics is carried out in the fish environment DNA dynamic water fate characteristic testing device; (3), on the basis of (2), an experiment on fish environment DNA degradation characteristics is carried out; (4), on the basis of (2), a test on fish environment DNA adsorption characteristics is carried out; (5), the process of environmental DNA concentration changing with time is tested and analyzed, and the fish environment DNA dynamic water fate characteristics are analyzed. According to the fish environment DNA dynamic water fate characteristic testing method and device, the fish environment DNA dynamic water fate characteristics of different types and in different stages of life history can be accurately and quantitatively tested, and the fish environment DNA dynamic waterfate characteristic testing method and device are of important scientific application value.

Description

technical field [0001] The invention relates to the field of water environment and water ecological protection, in particular to a method and device for testing the dynamic and water fate characteristics of fish environmental DNA. Background technique [0002] The impact of water conservancy projects on fish breeding habitats has attracted widespread attention, and monitoring and evaluation is an important basis for protection. As a non-invasive and time-sensitive emerging method, environmental DNA monitoring is widely used to judge the presence or absence of rare or invasive species. Studies have found that there is a correlation between the concentration of environmental DNA and the biological characteristics of fish and the environmental characteristics of water bodies, but trying to apply it to predict and evaluate the distribution of fish and their reproductive habitats, life history behavior, biomass, etc., still needs to solve how to test environmental DNA The bottle...

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Application Information

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IPC IPC(8): C12Q1/686C12M1/34G16B5/00
CPCC12Q1/686G16B5/00C12Q2561/113C12Q2563/107C12Q2545/114Y02A40/81
Inventor 郭辉杨文俊罗玉兰段文刚滕素芬黄明海李利黄卫
Owner CHANGJIANG RIVER SCI RES INST CHANGJIANG WATER RESOURCES COMMISSION
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