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Application of miR-1207 and target gene of miR-1207 in detecting laryngeal squamous cell carcinoma

A mir-1207 and target gene technology, applied in the application field of miR-1207 and its target gene in the detection of laryngeal squamous cell carcinoma, can solve the problem of few molecular markers

Active Publication Date: 2019-10-08
SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prognosis of laryngeal cancer is related to many factors, such as the degree of pathological differentiation of the patient, clinical stage, presence or absence of distant metastasis, and the choice of treatment, etc. Currently, there are relatively few effective molecular markers for the prognosis of laryngeal cancer

Method used

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  • Application of miR-1207 and target gene of miR-1207 in detecting laryngeal squamous cell carcinoma
  • Application of miR-1207 and target gene of miR-1207 in detecting laryngeal squamous cell carcinoma
  • Application of miR-1207 and target gene of miR-1207 in detecting laryngeal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening of biomarkers associated with laryngeal squamous cell carcinoma

[0043] 1. Sample collection

[0044] Six cases of laryngeal squamous cell carcinoma tissues and corresponding paracancerous tissue samples were collected for high-throughput sequencing.

[0045] 2. RNA sample preparation and quality analysis

[0046] Use the TRIZOL method to extract tissue RNA, the steps are as follows:

[0047] 1) Pre-cool the mortar with liquid nitrogen, put the tissue sample into the mortar with liquid nitrogen, and fully grind the tissue sample into powder under liquid nitrogen.

[0048] 2) Transfer the sample powder into a 2.0mL EP tube filled with TRIzol lysate, shake vigorously, mix well, and let it stand at room temperature for 5-10min.

[0049] 3) Centrifuge at 10000 rpm at 4°C for 5 minutes.

[0050] 4) Pipette the supernatant into a new 2.0mL EP tube, add 200μL of chloroform / isoamyl alcohol to each mL of the lysate, and mix vigorously by inversion.

[0...

Embodiment 2

[0088] Example 2 QPCR detection of expression of miRNA and its target genes in laryngeal squamous cell carcinoma samples

[0089] 1. Carcinoma tissue samples and paracancerous tissue samples of 29 laryngeal squamous cell carcinoma patients collected according to the collection method in Example 1 were subjected to large sample QPCR verification of differentially expressed genes.

[0090] 2. Extraction of RNA

[0091] Use the TRIZOL method to extract tissue RNA, and the specific steps are the same as Example 1.

[0092] 3. cDNA synthesis by reverse transcription

[0093] 3.1 Synthesis of lncRNA cDNA by reverse transcription

[0094] mRNA reverse transcription was performed using the FastQuant cDNA First Strand Synthesis Kit (Product No.: KR106).

[0095] 1) To remove the genomic DNA reaction, add 2.0 μl of 5×gDNA Buffer, 1 μg of TotalRNA, and RNase Free ddH to the test tube 2 O to bring total volume to 10 μl.

[0096] 2) Heat in a water bath at 42°C for 3 minutes.

[0097...

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Abstract

The invention relates to the application of miR-1207 and a target gene of the miR-1207 in detecting laryngeal squamous cell carcinoma. A high-throughput sequencing method is adopted to find genes withsignificant differential expression in laryngeal squamous cell carcinoma and adjacent tissues, and bioinformatics analysis is combined to select miR-1207-3p and the target gene KC6 and LINC00319 of the miR-1207 from alternative miRNA for RT-PCR verification, and the result shows that miR-1207-3p and the target gene of the miR-1207-3p have a good correlation with the laryngeal squamous cell carcinoma. The application of miR-1207 and the target gene of the miR-1207 in detecting laryngeal squamous cell carcinoma provide a novel important target for clinical diagnosis and treatment of the laryngeal squamous cell carcinoma.

Description

technical field [0001] The invention relates to the field of molecular diagnosis, in particular to the application of miR-1207 and its target gene in detecting laryngeal squamous cell carcinoma. Background technique [0002] Laryngeal cancer is one of the common malignant tumors of head and neck squamous cell carcinoma, most of which are squamous cell carcinoma (Laryngeal squamous cell carcinoma, LSCC) (Chu EA, Kim YJ. Laryngeal cancer: diagnosis and preoperative work-up [J]. Otolaryngol Clin NorthAm, 2008, 41 (4): 673-95.), this pathological type is quite common, and the incidence rate ranks sixth among all cancers. The incidence of laryngeal cancer ranks second among head and neck tumors, accounting for about 1 to 5% of all tumors in the whole body. Research and surveys at home and abroad show that the increase in the incidence of laryngeal cancer has been at a high level. [0003] The occurrence of laryngeal squamous cell carcinoma is related to many factors, such as smo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178Y02A50/30
Inventor 崔晓峰陈向军张思林
Owner SHENZHEN HOSPITAL OF SOUTHERN MEDICAL UNIV
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