Method for measuring inorganic arsenic content of marine algae products

Abstract

The invention discloses a method for measuring inorganic arsenic content of marine algae products. The method comprises the following steps: A, digestion: weighing smashed and dry algae products to bedetected, placing the algae products to be detected in a first centrifugal tube, adding nitric acid and shaking uniformly to obtain a mixed solution; B, extraction: treating the mixed solution in a water bath at a constant temperature, cooling the mixed solution to normal temperature, centrifuging the mixed solution in a centrifugal machine, placing upper supernatant in a second centrifugal tube,adding normal hexane and centrifuging in the centrifugal machine; C, purification: placing lower supernatant in the second centrifugal tube into a third centrifugal tube after centrifugation, addingmixed powder of active carbon and C18, performing vortex oscillation, centrifugating in the centrifugal machine, and placing the supernatant of the third centrifugal tube after centrifugation into anorganic filtering membrane for filtering to obtain a sample solution to be tested; and D, test: quantitatively testing the sample solution to be detected with a liquid chromatogram atomic fluorescencespectrometer. The detection method has the advantages of high accuracy, convenience and rapidness in operation method and high detection efficiency.

Description

technical field [0001] The invention relates to the technical field of food testing, in particular to a method for measuring the content of inorganic arsenic in seaweed products. Background technique [0002] In aquatic organisms (including animals and plants), arsenic exists in complex and diverse forms, mainly including organic arsenic and inorganic arsenic, of which organic arsenic is mostly non-toxic or less toxic, while inorganic arsenic [including As(III) and As(V)] have high toxicity and carcinogenicity, so the determination of the content of inorganic arsenic can basically be used as a standard for evaluating toxic arsenic in aquatic products. Aquatic organisms have a strong ability to accumulate arsenic, especially highly toxic inorganic arsenic. Therefore, accurate and rapid detection of inorganic arsenic in aquatic products has attracted widespread attention, and it is especially important for people's health and safety. important. [0003] In the prior art, dom...

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Problems solved by technology

GB 5009.11-2014 "National Food Safety Standard Determination of Total Arsenic and Inorganic Arsenic in Food" came into effect on March 21, 2016, replacing the old standard GB / T 5009.11-2003 "Determination of Total Arsenic and Inorganic Arsenic in Food" However, the determination method of inorganic arsenic content specified in the new standard is only applicable to the determination of inorganic arsenic content in rice, aquatic animals, cereal supplementary food for infants and young children, and canned supplementary food for infants and young children, and is not applicable to aquatic plants, especially seaweeds. Determination of Inorganic Arsenic Content in Products

At present, the determination of inorganic arsenic content in seaweed products needs to be purified by C18 immunoaffinity column, but in the actual purification process, there are still low filtration efficiency and the interference of pigments cannot be eliminated, which is very important for large quantitative instruments. big damage

In addition, studies have found that the chromatographic peak of arsenobetaine in seaweed products often covers the chromatographic peak of trivalent arsenic, which makes it impossible to accurately quantify the content of trivalent arsenic in seaweed products

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